高效液相色谱手性流动相法拆分布洛芬对映体

High Performance Liquid Chromatographic Separation of Ibuprofen Enantiomer with Chiral Mobile Phase Additives

目的:建立 HPLC 手性流动相法拆分布洛芬对映体。方法:选用 Agilent Eclipse XDB-C_8柱(4.6mm×250mm,5μm);Lab Alliance C_8保护柱(4.6mm×10mm);流动相:水-甲醇-乙腈(78:17:5,含80.0 mmol·L^(-1)羟丙基-B-环糊精、0.2%磷酸,pH 4.50);流速:1.0mL·mm^(-1);紫外检测波长:265nm,进量样:20μL;柱温:室温。结果:建立了羟丙基-β-环糊精动态手性流动相法分离布洛芬对映体的方法。结论:该方法分离度好,简便,且比手性固定相经济。

Objective:To establish HPLC chiral mobile phase for resolution of ibuprofen enantiomer.Methods:Agi- lent Eclipse XDB-C_8 column(4.6 mm×250 mm,5μm),with the mobile phase consisted of H_2O-CH_3OH- CH_3CN(78:17:5,containing 80.0 mmol·L^(-1)HP-β-CD and 0.2% H_3PO_4,pH 4.50),flow rate of 1.0 mL· min^(-1),detection wavelength at 265 nm.Result:HP-β-CD chiral mobile phase method resolution of ibuprofen en- antiomer was established.Conclusion:This technique leads to column with excellent time stability and good repro- ducibility of the enantio-selectivity.