液相色谱法测定强力救心滴丸中蟾酥的华蟾酥毒基和酯蟾毒配基含量

Quantitative Determination of Venenum Bufonis in Qiangli Jiuxin Dripping Pills by HPLC

目的:测定强力救心滴丸中蟾酥的主要成分华蟾酥毒基和酯蟾毒配基含量。方法:样品经甲醇超声处理提取,采用高效液相色谱法测定,色谱柱为 Alltima C_(18)色谱柱(4.6mm×250mm,5μm);柱温:40℃;流速:1mL·min^(-1);流动相:乙腈-0.5%磷酸二氢钾(pH=3.2)(50:50);检测波长:296nm。结果:华蟾酥毒基在0.25-1.51μg范围内、酯蟾毒配基在0.26-1.55μg范围内具有良好线性关系。华蟾酥毒基平均加样回收率为99.6%,RSD=2.1%(n=5);酯蟾毒配基平均加样回收率为98.2%,RSD=1.4%(n=5)。结论:该法准确、可靠,可用于强力救心滴丸中蟾酥的酯蟾毒配基和华蟾酥毒基含量测定。

Objective:Determine the content of Venenum Bufonis in Qiangli Jiuxin dripping pills.Methods:The sample is ultrasonic extracted in methanol.With HPLC,the column is Alltima C_(18)(4.6 mm×250 mm,5 μm),the column temperature is 40℃,the flow rate is 1 mL·min^(-1),the mobile phase is acetonitrile-0.5% potassium di- hydrogen phosphate(pH=3.2)(50:50);The detective wavelength is 296 nm.Results:The linear range of cinobu- fagin is 0.25-1.51 μg,the average recovery is 99.6% and RSD 2.1% (n=5).The linear range of resibufogenin is 0.26-1.55 μg,with the average recovery of 98.2% and RSD 1.4% (n=5).Conclusion:The method can be applied to determination of resibufogenin and cinobufagin in Qiangli Jiuxin dripping pills.