分光光度法测定甘草中总黄酮的含量

Determination of Total Flavone in Licorice by Spectrophotometry

目的:建立分光光度法测定甘草中总黄酮含量。方法:利用二氢黄酮类在碱性条件下易转化为它的相应异构体-查尔酮类化合物特性,采用分光光度法,以甘草苷为对照品,以10%氢氧化钾为显色剂,在波长为400nm 处对样品中的总黄酮进行含量测定。结果:总黄酮在2.05-41.13μg·mL^(-1)范围内呈良好的线性关系(r=0.9992);该法的平均加样回收率分别为97.6%,RSD 分别为2.0%(n=3)。结论:该方法稳定、简便、快速,适用于甘草中总黄酮含量的测定。

Objective:A method for determination of total flavone in Licorice was set up.Method :Using the charac- teristic that flavanonols are easily transformed chalcones under alkali conditions.Reference substance was liquiritin, 10% KOH was chromogenic agent and the detection wavelength was at 400nm.Result:The linear range was be- tween 2.05-41.13μg·mL^(-1)(r=0.9992)and the average recovery was 97.6% with corresponding RSD of 2.0%(n=3).Conclusion:This method is simple,convenient,rapid and is suitable for determination of total fla- vone in Licorice.