五味子乙素对M146L细胞分泌β淀粉样蛋白的影响

Inhibition of γ-schisandrin on the production of amyloid β-protein from M146L cells

目的:探讨五味子乙素对M146L细胞分泌的β淀粉样蛋白(amyloid β-protein,Aβ)的影响。方法:体外培养稳定转染人类阿尔茨海默病β淀粉样前体蛋白(Amyloid beta-protein precursor,APP)基因及突变型早老素1(prsenilin-1,PS1)基因的CHO(Chinese Hamster Ovary)细胞系(M146L),使之高效产生β淀粉样蛋白42(amyloid β-protein,Aβ_(42)),建立Aβ_(42)过度表达的细胞模型。加入待筛选的药物五味子乙素,用四甲基偶氮唑蓝比色法(microculture tetrazolium assay,MTT法)检测不同浓度的五味子乙素(1.67,5,15μg·mL^(-1))对M146L细胞毒作用,应用ELISA法(enzyme-linked immunosorbent assay)观察细胞分泌的Aβ_(42)的变化。结果:不同浓度的五味子乙素对M146L存活率没有影响,不具有细胞毒作用。5和15μg·mL^(-1)的五味子乙素对细胞分泌的Aβ_(42)有抑制作用,以高剂量组最为明显。结论:一定剂量的五味子乙素对M146L细胞分泌的Aβ_(42)有明显的抑制作用。

Objective: To study the effects ofγ-schisandrin on the production of amyloid β-protein 42 (Aβ_(42)) from M146L cells.Methods:Chinese hamster ovary (CHO) cells (M146L) that stably overexpress amyloid β-protein precursor for Aβ_(42) and mutant presenilin (M146L) were firstly established in vitro, and then supplemented with γ-schisandrin. The microculture tetrazolium assay (MTT assay) and enzyme-linked immunosorbent assay (ELISA) were used to evaluate the effects of γ-schisandrin (1.67,5 and 15 μg·mL-1) on Aβ_(42) production in the cell model. Results: γ-Schisandrin showed no apoptosis effects on M146L cells in MTT assay, and inhibited the Aβ_(42) production at concentrations of 5 and 15 μg·mL^(-1) in the ELISA assay. Conclusion:γ-Schisandrin potentiates to be the inhibitor of Aβ_(42) production.