宫颈癌介入化疗前后核形态、AgNOR的定量研究

Quantitative Study on Nuclear Morphometry, AgNOR of Cervical Cancer with Intra-Arterial Infusion Chemotherapy

目的探讨介入化疗对宫颈鳞癌细胞核形态、核仁组成区嗜银蛋白(AgNOR)的影响。方法用HE和嗜银染色对30例进行介入化疗前后的宫颈癌组织染色,并用真彩色图像分析系统测量肿瘤细胞的核面积、核周长、最大直径、AgNOR颗粒截面数、颗粒直径、颗粒总面积、颗粒总面积/核面积。结果介入后宫颈癌细胞的核面积、核周长、核最大直径较介入前缩小,P均<0.05,差异有显著性;介入前后核体积密度、核数密度、表面积密度比较P均<0.05,差异有显著性;介入前后核平均体积、核平均表面积、三维形状因子比较,P均<0.05,差异有显著性;介入后AgNOR颗粒截面数、颗粒总面积、颗粒直径、颗粒总面积/核面积较介入前减少或缩小,P均<0.05,差异有显著性。结论介入化疗可以缩小宫颈癌细胞的核面积、核周长,并且介入化疗前后宫颈癌的组织结构参数(VV、SV、NV,V、S、Rsv)存在显著差异;介入化疗可以减少AgNOR颗粒截面数,可以抑制宫颈癌细胞的增殖。

Objective To study the effect of intra-arterial infusion chemotherapy on nuclear morphometry, AgNOR in cervical cancer.Methods Thirty cervical cancers were examined for multiple biomarkers(nuclear morphometry, AgNOR) with quantitative compute processed imaging analysis before and after intra-arterial infusion chemotherapy.Results Statistical analysis showed that there were significant differences between the groups(P<0.05). (1)There was significant differences in 9 parameters(Prmtr,At,Vv,Nv,Sv, Rsv) between before and after intra-arterial infusion chemotherapy of nuclear morphometry.(2)AgNOR counts of before and after intra-arterial infusion chemotherapy were(4.97±0.85)μm and(3.80±0.71)μm. After treatment, the AgNOR configuration changed greatly.Conclusion Intera-arterial infusion chemotherapy can inhibit the proliferation of cervical cancer cell. It is recommended that intra-arterial infusion chemotherapy be employed to be one of combined treatments for cervical cancer.