视神经脊髓炎的CD4^+T细胞内细胞因子的分析

Analysis of Intracellular Cytokines of CD4^+ T Cells in Neuromyelitis Optica

目的探讨CD4+T细胞内细胞因子在视神经脊髓炎及其临床分期中的特点及变化。方法对视神经脊髓炎和健康对照的末梢血中CD4+T细胞内的细胞因子染色并利用流式细胞仪进行测定和分析。结果与健康对照比较视神经脊髓炎CD4+IFNγ+IL4-细胞在复发期和缓解期均显著增加。结论视神经脊髓炎是以I型辅助性T细胞的反应占优势,在其复发期和缓解期这种反应的优势均明显提高。同时也说明利用流式细胞仪对T细胞亚型进行测定在神经系统自身免疫性疾病的分析过程中有一定的参考价值。

Objective To analyze intracellular cytokines of CD4 + T cells in neuromyelitis optica (NMO) during relapse phase and remission phase. Methods The intracellular cytokines of CD4 + T cells were examined by flow cytometry in peripheral blood from patients with NMO. Healthy subjects were also examined as controls. Results Patients with NMO during relapse and remission phases showed a significantly higher percentage of CD4 +IFN-γ +IL-4 -. Conclusion Type I help T cells (Th1) response is predominant in NMO. The predominant showed a significantly higher in the relapse phase and remission phase of NMO than the healthy. Our findings therefore suggest a flow cytometry analysis of T cells subsets to be useful for differentiating autoimmune disease of nervous system.