摘要
构建了一种亲和素单分子层修饰的电化学分析生物芯片基片,并且以二茂铁和亚铁氰化钾为指示剂对该基片的电化学性能进行了研究.循环伏安实验结果表明, 单分子层的存在增强了二茂铁在基片上的响应电流,二茂铁可以作为该基片的高效电子媒介体,实验结果具有良好的重现性和一致性,基片产生的背景噪声可以忽略不计.文中对这些结果产生的原因进行了讨论.
A biotin-avidin modified biochip has been constructed, and its electrochemistry characteristics were studied with K_4Fe(CN)_(6).3H_2O and ferrocene as the indicators by cyclic voltammograms. Electrochemical experiments showed that this biochip has high response signal, reproducibility,uniformity and low background signal; the ferrocene can be used as high efficiency electron medium. And these results were discussed in this paper.
出处
《武汉大学学报（理学版）》
CAS
CSCD
北大核心
2005年第2期249-252,共4页
Journal of Wuhan University:Natural Science Edition
基金
国家863计划资助项目(2001AA514020 02)
