饲予克山病病区粮对大鼠胰岛β细胞分泌功能及胰岛素、C肽分泌贮备的影响

EFFECTS ON INSULIN AND C PEPTIDE CONCENTRATION IN SERUM AND ISLETS OF RATS FED GRAINS FROM A KESHAN DISEASE ENDEMIC AREA

应用免疫组织化学及放免分析方法,对饲予克山病病区粮大鼠胰岛的形态与机能研究结果表明:低硒的克山病病区粮可引起大鼠血清胰岛素、C肽水平明显下降,胰岛内C肽和胰岛素分泌贮备显著减少,并出现胰岛β细胞萎缩、变性等形态学损害。在病区粮内补一定剂量硒或维生素E(VE)可明显提高血清胰岛素、C肽水平,增加β细胞分泌贮备,提示硒和VE缺乏引起的过氧化损害可能是胰岛受损的主要机制。本研究结果提示克山病基本病因可引起胰岛的原发性损害,其作为克山病发病过程中的重要环节,可能对心肌坏死的发生发展起一定作用。

The islets function and morphology of rats fed grains from a Keshan Disease Endemic area were studied by immunohistochemistry and R1A methods. The results demonstrated insulin and C peptide concentration reduced and insulin, C peptide secretory reserve in islets also decreased in endemic grains group,simultaneously the LPO contents and GSH-Px activity in serum and pancreas respectively increased and decreased in the rats. There were lesions of islets on morphology in the rats. The diets that supplemented the grains with either selenium (0. 2 mg/kg dietlor vitamin E(500mg/kg) caused the increase of concentration of insulin and C peptide in serum and pancreas,the GSH-Px activity increased and the LPO contents decreased in serum and pancreas of the rats. The study suggested the Keshan Disease and its related factors may cause the lesion of islets, which may aggravate myocardium lesions in Keshan Disease.