金不换药材的含量测定

Content Determinantion of Radix Stephaniaepiqaea by Utraviolet Spectrophotometry

目的:建立金不换药材含量测定方法。方法:用紫外分光光度法测定为该药材中延胡索乙素含量,检测波长为281 nm。结果:3批金不换药材干燥品平均含量在1.49～1.52 mg·g-1内,延胡索乙素线性范围16.4-41.0μg·ml-1,线性方程:A =0.0160 C+0.017 6,相关系数r=0.999 5,平均回收率95.8%。结论:用紫外分光光度法测定延胡索乙素含量对于金不换药材质量控制来说具有快速、准确的特点。

Objective: To establish a better method to determine the content of radix stephaniaepiqaea. Method: The content of dl-tetrahydropalmatine was determined by ultraviolet spectrophotometry, the detecting wavelength was at 281 nm. Result: The linear range was within 16.4-41.0 μg·ml-1.The average recovery of dl-tetrahydropalmatine was 95.8% , Conclusion: The method is rapid,accurate, and suitable for the quality control.