FQ-PCR检测非淋菌性尿道炎中沙眼衣原体和解脲支原体

Detect the Gene of CT and UU of Non-gonococcd Urthritis by Fluorescence Quantitative PCR

目的研讨FQ-PCR技术在了解CT、UU的感染情况,为临床诊断和治疗提供科学依据方面的实践价值。方法应用FQ-PCR技术检测男女生殖道标本780份。其中男性320份,女460份。结果UU阳性率为30.13%(235/780),其中男性阳性率11.56%(37/320),女性阳性率43.04%(198/460)。CT阳性率10.64%(83/780),其中男性阳性率9.68%(31/320),女性阳性率11.30%(52/460)。UU感染率在男女性别之间有显著性差别(P<0.01),CT感染率在性别之间无显著差别(P>0.05)。结论FQ-PCR技术检测CT、UU基因具有简便、快速、特异性高、定量准确等特点。为临床诊断、治疗提供了快速准确的科学依据。

Objective To detect quantitotively the of Ureaplasma urealyticum(UU) and chlamydia trachomatis(CT) in non-genococcal urethritis by technoligy of fluorescence quantitalive polymerase chain reaction (FQ-PCR),to understand further infections condition of UU and CT ,and to provide clinical treatment evidence to the disease. Methods Detect 780 speciments by techmology of FQ-PCR of all these, 320 speciments were male and 460 specients were female. Results The positive rate of UU was 30.13%(235/782) of all these ,male positive rate of CT was 11.56%(37/320) and female was 43.04%(198/460), The positive rate of CT was10.64%(83/780) of all these,male positive rate was 9.68%(31/320) and female was 11.30%(52/460). infections rate of UU had obvious statistics differnce between males and females(P<0.01),but CT hadn't(P>0.05). Conclusion Technology of FQ-PCR is simple, rapid, excellent and accurafe. FQ-PCR have directive meaning for the dagnosis and trentment.