摘要
将具有广谱拮抗作用的枯草芽孢杆菌(Bacillussubtilis)H110作为生防菌株,研究了该菌的培养液、滤液、菌悬液及蛋白粗提液对苹果梨(PyrusbretschneideriRehd. )青霉病(PenicilliumexpansumLink)和黑斑病(Alternariaalternate)的抑制效果.H110的蛋白粗提液的抑制作用最好,青霉病和黑斑病的发病率分别比对照低92. 7%和86. 6%,病斑直径也显著小于对照;其次为菌培养液和菌悬液,滤液的抑制效果相对较差,但4种处理均显著好于对照. 4种处理对病原菌孢子萌发的抑制效果与invivo试验的效果一致.实验结果表明,枯草芽孢杆菌通过产生抗菌蛋白和竞争作用抑制病原菌生长.
Bacillus subtilis H110, with broad spectrum resistance to plant pathogens, was evaluated for its effectiveness in postharvest control of blue mold and black spot caused by Penicillium expansum and Alternaria alternata on apple-pear ( Pyrus bretschneideri Rehd.) fruit. Four formulations of B. subtilis all could significantly reduce blue mold and black spot incidence and severity at 25 ℃. Results from in vitro studies showed that these formulations could also greatly affect spore germination. Crude protein extraction was most effective for controlling the two diseases in these treatments. The mechanism by which B. subtilis H110 inhibited pathogens might be related to its ability to produce antagonistic protein and compete for nutrients and space. Fig 1, Tab 2, Ref 17
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2005年第2期171-174,共4页
Chinese Journal of Applied and Environmental Biology
关键词
枯草芽孢杆菌
苹果梨
青霉病
黑斑病
生物防治11[
Bacillus subtilis
Pyrus bretschneideri Rehd.
Penicillium expansum Link
Alternaria alternate
biocontrol
