3株真菌对毒死蜱的降解特性

DEGRADATION CHARACTERISTICS OF THREE FUNGI TO CHLORPYRIFOS

从污水排放口污泥中分离到3株以毒死蜱为唯一碳源生长的真菌WZ-Ⅰ、WZ-Ⅱ、WZ-Ⅲ,鉴定均为镰孢霉属(Fusarium LK.ex Fx).3株菌5 d内对50mg L-1毒死蜱的降解率分别高达93.5%、91.4%和83.5%.测定了不同碳源、pH、温度及毒死蜱浓度对真菌降解能力和生长量的影响.结果表明,以毒死蜱为唯一碳源且其浓度为20～200 mgL-1,pH 6.5～9.0,温度30～40℃时,真菌的降解效果较好;真菌生长量随外加碳源浓度的增加而增加,在pH 6.5～9.0时生长量较大,且当毒死蜱浓度为50 mg L-1,温度40℃时其生长量最大.

Three chlorpyrifos-degrading fungi, with chlorpyrifos as sole carbon source, were isolated from sludge at sewage outlet and identified as Fusarium LK. ex Fx. The degradation rates of chlorpyrifos by these fungi were 93.5%, 91.4% and 83.5% in 5 days,respectively. The effects of carbon source, pH, temperature and chlorpyrifos concentration on degradation were determined. The results showed that the biodegradation rates were higher when chlorpyrifos was the sole carbon source with concentration of 20~200 mg L -1 , pH 6.5~9.0 and temperature 30~40 ℃. The growth of the fungi increased with carbon source concentration increased, and was the highest when pH was 6.5~9.0, temperature 40 ℃ and chlorpyrifos concentration 50 mg L -1 . Fig 5, Tab 1, Ref 18