新疆布顿大麦(Hordeum bogdanii Wilensky)叶绿体基因组PCR-RFLP分析

PCR-RFLP analysis of chloroplast DNA among Hordeum bogdanii Wilensky accessions native to Xinjiang,China

利用7个叶绿体基因组(cpDNA)的PCR标记对32份新疆布顿大麦(HordeumbogdaniiWilensky)进行了分析。结果表明,在7个叶绿体基因组PCR标记中,均能得到1条清晰的扩增产物,且扩增产物直接电泳均无多态性。利用HinfⅠ、HhaⅠ、HaeⅢ和RsaⅠ等4种限制性内切酶消化后,在所有28种标记/酶组合中,共有2个标记(rbcL和trnS psbC)的6种酶组合(占21 4%)能检测到多态性。在28种标记/酶组合,共检测到83条扩增片段,其中14条(占16 9%)具有多态性。叶绿体基因组PCR RFLP标记揭示的32份新疆布顿大麦间遗传距离值变化范围为0～0 080,平均值为0 030。利用叶绿体基因组PCR RFLP标记遗传距离系数,采用UPGMA法构建了32份新疆布顿大麦间的遗传关系聚类图。结果表明,利用7个叶绿体基因组STS PCR标记的28种标记/酶组合不能将所有供试的32份新疆布顿大麦区分开。

The genetic diversity among 32 accessions of Hordeum bogdanii Wilensky native to Xinjiang, China was detected by using 7 chloroplast DNA (cpDNA) PCR markers.Among the 32 H.bogdanii accessions, all the 7 cpDNA PCR markers could produce one distinct band, and no polymorphism was detected by direct electrophoresis in 2 % agrose gels. Of the seven cpDNA PCR markers, only 2 markers (rbcL and trnS-psbC) and 6 marker/ enzyme combinations generated polymorphic bands upon digestion with HinfI,HhaI,HaeⅢ and RsaI,while 5 out of 7 markers (80.0 %) and 22 out of 28 marker/ enzyme combinations (78.6 %) did not reveal polymorphisms. A total of 83 bands were detected in 28 cpDNA PCR-RFLP marker/ enzyme combinations, among which 14 bands (16.9 %) were polymorphic. The cpDNA PCR-RFLP-based genetic diversity (GD) among 32 H.bogdanii accessions ranged from 0 to 0.080, with the mean of 0.030. Based on the GD matrix, a dendrogram showing the genetic relationships between accessions was constructed using the UPGMA method. The results showed that all 32 accessions could not be distinguished by 7 cpDNA PCR markers.