用SPF鸡制备IBD诊断抗原及血清的研究

Study on preparation of diagnostic antigen and serum for IBD using SPF chickens

将IBDV接种SPF鸡，死者取其法氏囊，经超声波粉碎或匀浆，反复冻融制备IBDAGP诊断原，未死者，再用IBD组织灭活苗免疫制备IBD诊断血清。如此制备的IBD诊断液特异性强、灵敏度高、成本低．适于禽病检验室推广应用。

Bursa were tuken from SPF chickens died with infection of infectious bursal disease virus and sonificated and homogenized,thus prepared as IBD AGP antigen. The survival SPF chickens were further immunized with inacti-vatal IBD vaccine for preperation of IBD serum. The dignodic reagents for IBD prepared with SPF chickens were both specific and sensible at a low cost,and can be widely used in avian diase laboratories.