摘要
研究了卡那霉素、潮霉素对浙江红山茶、大花红花油茶愈伤组织的生长及对攸县油茶幼苗叶片诱导愈伤的影响,确定作为选择压力的卡那霉素质量浓度为50～60mg·L-1,潮霉素为25～30mg·L-1。在3种抑菌性抗生素中,羧苄青霉素促进愈伤组织诱导,对愈伤组织的生长没有影响,头孢霉素则有抑制作用,而羟氨苄青霉素轻微抑制愈伤组织的生长,但对愈伤组织的诱导影响不大。考虑到抑菌效果,适于在转基因研究中使用的羧苄青霉素的抑菌质量浓度为250mg·L-1,羟氨苄青霉素为125mg·L-1。
The effect of the supplement of antibiotics on induction and growth of camellia callus was studied. Kannamycin and Hygromycin were more sensitive to growth of callus of C.chekiangoleosa than that of C.magniflora. Growth of callus would be limited by Kannamycin at 50～60 mg·L^(-1) and Hygromycin at 25～30 mg·L^(-1). The leaves of C.grijsii seeding could not induced callus when Kannamycin and Hygromycin arrived the same concentration. Carbenicillin could accelerate induction of callus at certain degree but didnot affect the growth of callus. Amoxicillin inhibited slightly the growth of callus. Cefotaximes reduced both the ratio. According to the effect of inhibiting Agrobacterium tumefaciens, concertration of Carbenicillin and Amoxicillin were 250 mg·L^(-1) and 125 mg·L^(-1) respectively in the study of transformation.
出处
《林业科学研究》
CSCD
北大核心
2005年第2期183-186,共4页
Forest Research
基金
中国林业科学研究院亚热带林业研究所青年科技发展基金项目"农杆菌介导茶花转化受体系统的建立"(C02 02)
