期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

血红素氧合酶-1在胰岛素对乳鼠心肌细胞保护机制中的作用 被引量：3

Role of heme oxygenase-1 in myocardial protective mechanism of insulin
下载PDF
导出
摘要 目的探讨血红素氧合酶(HO)1在胰岛素对缺氧/复氧心肌细胞保护机制中的作用。方法体外培养SD乳鼠心肌细胞,分为7组常氧对照组,缺氧/复氧对照组,不同剂量胰岛素组(160、320、640、960mU/L),胰岛素加原卟啉锌组(胰岛素640mU/L、原卟啉锌10μmol/L)。除常氧对照组外,其余各组均给于缺氧3h,再复氧1h处理。检测心肌细胞HO1蛋白表达、HO活性、细胞生存率及乳酸脱氢酶(LDH)值。结果与缺氧/复氧对照组比较,各胰岛素组心肌细胞HO1蛋白表达显著增加,HO活性、细胞生存率显著增高,LDH显著降低。原卟啉锌能显著抑制HO活性,阻止胰岛素对心肌细胞的保护作用。结论胰岛素能够增加缺氧/复氧心肌细胞HO1的表达,增高HO活性,从而减轻心肌细胞缺氧/复氧损伤。 AIM: To explore the role of heme oxygense-1(HO-1) in the myocardial protection of insulin. METHODS: Neonatal cardiomyocytes of SD rats cultured in vitro, and then the study was performed according to different groups: Normoxia; Hypoxia-reoxygenation; Insulin(160, 320, 640, 960 mU/L); Insulin(640 mU/L) and Zinc Protoporphyrin IX (znpp,10 μmol/L). Except Normoxia, the other groups were subjected to 3 hours of hypoxia and 1 hour of reoxygenation. Expression of HO-1,HO activity, cell viability and lactate dehydrogenase (LDH) were detected. RESULTS: In comparison with control group, HO-1 expression, HO activity and cell viability increase, however, LDH levels decreases in insulin groups. Znpp could inhibit HO activity and blockade the myocardial protective mechanism of insulin. CONCLUSION: Insulin could reduce the hypoxia-reoxygenation injury of cardiomyocytes by increasing HO-1 expression and HO activity.
作者 李锦 蒋晖 毛建斌 陈茂 范忠才 黄德嘉
机构地区 四川大学华西医院心内科
出处 《心脏杂志》 CAS 2005年第2期111-114,共4页 Chinese Heart Journal
关键词 胰岛素 心肌细胞 血红素氧合酶-1 缺氧/复氧损伤 insulin cardiomyocytes heme oxygenase-1 hypoxia-reoxygenation injury
分类号 Q463 [生物学—生理学]
  • 相关文献

参考文献10

  • 1Diaz R, Paolasso EA, Piegas L.S, et al. On behalf of the ECLA ( Estudios Cardiologicos Latinoamerica) collaborative group. Metabolic modulation of acute myocardial infarction: the ECLA glucose-insulinpotassium pilot trial [J]. Circulation, 1998, 98 ( 21 ): 2227 -2234.
  • 2杨萍萍,袁文俊.血红素氧化酶-1对心脏的保护作用[J].心脏杂志,2003,15(4):375-377. 被引量：5
  • 3Das UN. Insulin: an endogenous cardioprotector [J]. Curr Ophn Crit Care, 2003, 9(5): 375 -383.
  • 4Melo LG, Agrawal R, Zhang L, et al. Gene therapy strategy for long-term myocardial protection using adeno-associated virus-mediated delivery of heme oxygenase gene [J]. Circulation, 2002, 105:602 - 607.
  • 5Turcanu V, Dhouib M, Poindron P. Determination of heme oxygenase activity in murine macrophages for studying oxidative stress inhibitors [J]. Analytical Biochomistry, 1998, 263:251-253.
  • 6Lee PJ, Jiang BH, Chin BY, et al. Hypoxia-inducible factor-1 mediates transcriptional activation of the heme oxygenase-1 gene in response to hypoxia [J]. J Biol Chem, 1997, 272(9): 5375 -5381.
  • 7Treins C, Giorgetti-Peraldi S, Murdaca J, et al. Insulin stimulates HIF-1 through a PI 3-kinase/TOR-dependent signaling pathway [J].J Biol Chem, 2002, 277(31): 27975-27981.
  • 8Akamatsu Y, Haga M, Tyagi S, et al. Heme oxygenase-1-derived carbon monoxide protects hearts from transplant associated ischemia reperfusion injury [J]. FASEB J, 2004, 18(6): 771 -772.
  • 9Gao F, Gao E, Yue TL, et al. Nitric oxide mediates the antiapoptotic effect of insulin in myocardial ischemia-reperfusion: the roles of PI3-kinase, Akt, and endothelial nitric oxide synthase phosphorylation [J]. Circulation, 2002, 105(12): 1497-1502.
  • 10Foresti R, Motterlini R. The heme oxygenase pathway and its interaction with nitric oxide in the control of cellular homeostasis [J].Free Radic Res, 1999, 31(6): 459 -475.

二级参考文献16

  • 1Yachie A, Niida Y, Wada T, et al. oxidative stress causes enhanced endothelial cell injury in human heme oxygenase-1 deficiency[J]. J Clin Invest,1999, 103:129--135.
  • 2Schipper HM, Chertkow H, Mehindate K, et al. Evaluation of heme oxygenase-1 as a systemic biological marker of sporadic AD[J]. Neurology,2000, 54:1297--1304.
  • 3Paredi P, Biernacki W, Invernizzi G, et al. Exhaled carbon monoxide levels elevated in diabetes and correlated with glucose concentration in blood : a new test for monitoring the disease? [J]. Chest, 1999,116:1007--1111.
  • 4Yer SF, Perrella MA, Layne MD,et al. Hypoxia inducie severe right ventricular dilatation and infarction in heme oxygenase-1 mull mice[J]. J Clin Invest,1999, 103:23--29.
  • 5Yet SF, Tian R, Layne MD, et al, Perrella Mal Cardiac-specif-ic expression of heme oxygenase-1 protects against ischemia and reperfusion injury in transgenic mice[J]. Circ Res, 2001, 89:168--173.
  • 6Hangaishi M, Ishizaka N, Aizawa T, et al. Induction of heme oxygenase-1 can act protectively against cardiac ischemia/reperfusion in vivo[J]. Biochem Biophys Res Commun, 2000, 279 : 582 --588.
  • 7Clark JE, Foresti R, Sarathchandra P, et al. Heme oxygenase- 1-clerived bilirubin ameliorates postischemic myocardial dysfunction[J]. Am J Physiol,2000, 278:H643--H651.
  • 8Christou H, Morita T, Hsieh CM, et al.Prevention of hypoxiainclued pulmonary hypertension by enhancement of endogenous heine oxygenase-1 in the rat [J]. Circ Res, 2001,86:1224-- 1229.
  • 9Sato K, Balls J, Otterbein L, et al. Carbon monoxide generated by heme oxygeusse-1 suppresses the rejection of mouse-to-rat cardiac transplants[J]. J Immuol, 2001,166:4185--4194.
  • 10Dore S, Takahashi M, Ferris CD, et al. Bilirubin, formed by activation of heme oxygenase-2, protects neurons against oxidative stress injury[J]. Proc.Nall Acad Sci USA, 1999,6:2445-- 2450.

共引文献4

同被引文献39

  • 1李培建,李兵仓.一种重组腺病毒载体的扩增、纯化和病毒滴度检测方法[J].中华神经医学杂志,2002,1(1):52-55. 被引量：15
  • 2Grauss RW, Winter EM, van Tuyn J, et al. Mesenchymal stem cells from ischemic heart disease patients improve left ventricular function after acute myocardial infarction[ J]. Am J Physiol Heart Circ Physiol, 2007, 293 (4) :H2438 - H2447.
  • 3Baron F, Gothot A. Mesenehymal stem cells: a new versatile therapeutic option[ J]. Rev Med Liege, 2007, 62(Spec No) :9 - 14.
  • 4Potier E, Ferreira E, Meunier A, et al. Prolonged hypoxia concomitant with serum deprivation induces massive human mesenchymal stem cell death[J]. Tissue Eng, 2007, 13(6) :1325 -1331.
  • 5Angermayr B, Mejias M, Gracia-Sancho J, et al. Heme oxygenase attenuates oxidative stress and inflammation, and increases VEGF expression in portal hypertensive rats[J]. J Hepatol, 2006, 44(6) : 1033 - 1039.
  • 6Tang YL, Tang Y, Zhang YC, et al. Improved graft mesenchymal stem cell survival in ischemic heart with a hypoxia-regulated heme oxygenase-1 vector[J]. J Am Coll Cardiol, 2005, 46(7):1339 - 1350.
  • 7Barbagallo I, Tibullo D, Di Rosa M, et al. A eytoprotective role for the heme oxygenase-1/CO pathway during neural differentiation of human mesenchymal stem cells[ J ]. J Neurosci Res, 2008, 86 (9) : 1927 - 1935.
  • 8Vorburger SA, Hunt KK. Adenoviral gene therapy[ J]. Oncologist, 2002, 7(1) :46 -59.
  • 9Liu X, Pachori AS, Ward CA, et al. Heme oxygenase-1 (HO-1) inhibits postmyocardial infarct remodeling and restores ventricular function[J]. FASEB J, 2006, 20(2) :207 -216.
  • 10Tang JM, Xie QY, Pan GD, et al. Mesenchymal stem cells participate in angiogenesis and improve heart function in rat model of myocardial ischemia with reperfusian [ J ]. Eur J Cardiothorac Surg, 2006, 30(2) :353 -361.

引证文献3

二级引证文献3

心脏杂志
2005年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部