挥发性有机化合物对小鼠神经行为功能的影响

Neurotoxic Effect of Exposure of Mice to Volatile Organic Compounds

[目的]研究室内挥发性有机化合物(VOCs)对小鼠神经行为的影响。[方法]用分析纯试剂配制成混合物,对小鼠用静式染毒柜进行亚慢性吸入染毒(3个剂量组分别是,甲醛:1.5、4.5、13 .5mg/m3 ;VOCs :2 0 .0、60 .0、180 .0mg/m3 )。观察小鼠一般情况,进行神经行为功能测试包括水迷宫、避暗、跳台、自发活动试验。[结果]染毒期间可见各染毒组小鼠活动减少,行动迟缓;雌性小鼠高剂量染毒组体重增长低于对照组,差异有显著性(P <0 .0 5 ) ;而在水迷宫、避暗、自发活动测试中,除雄性小鼠低剂量组与对照组差异无显著性外,其余雌、雄性小鼠各染毒组与对照组相比,结果差异均有显著性(P <0 .0 5 )。雄性小鼠体重增长,差异未见显著性。[结论]挥发性有机化合物可使小鼠体重减轻,活动减少,学习记忆能力下降。

Objective] To explore the combined neurotoxicity of volatile organic compounds(VOCs) exposure in mice. [Methods] The gaseous mixture of several main compounds of VOCs and formaldehyde were prepared using their pure chemical reagents.The Kunming mice were sub-acutely exposed to the prepared gaseous mixture(three groups of formaldehyde at a dose of 1.5 mg/m3,4.5 mg/m3 or 13.5 mg/m3;three groups of VOCs at a dose of 20 mg/m3,60 mg/m3 or 180 mg/m3).Neurobehavioral function tests include spatial water maze,step-down test,step-through test and pontaneous activity measurement. [Results] Significant differences were observed in all tests between exposed groups and the control group. [Conclusion] It is suggested that volatile organic compounds exposure could damage the ability of learning and memory in mice.