地塞米松对哮喘患者外周血单个核细胞p38MAPK表达的影响

Effect of Dexamethasone on p38 Mitogen-activated Protein Kinase in Peripheral Blood Mononuclear Cells in Patients with Bronchial Asthma

目的探讨支气管哮喘患者外周血单个核细胞(PBMCs)p38MAPK表达的变化及地塞米松(DEX)对其的影响。方法分离培养哮喘患者和正常健康者的PBMCs。分别采用蛋白质印迹和ELISA方法检测PBMCs核蛋白p38MAPK的表达及细胞上清液中IL -4、IL- 5的蛋白质浓度。结果哮喘对照组PBMCs核蛋白p38MAPK表达及细胞上清液中IL -4、IL- 5的蛋白质浓度较正常对照组显著升高(P<0. 001),DEX处理组核蛋白p38MAPK表达及细胞上清液中IL- 4、IL- 5的蛋白质浓度较哮喘对照组显著降低(P<0. 01)。通过直线相关分析发现,PB MCs核蛋白p38MAPK表达与培养上清液中IL- 4、IL- 5蛋白质含量之间均呈显著正相关(r分别=0. 71、0. 64,P均<0. 01)。结论p38MAPK可能参与支气管哮喘的病理生理过程。DEX可能通过作用于p38MAPK这一靶点发挥其抗炎效应。

Objective To study the changes of p38 mitogen-activated protein kinase(p38 MAPK) in peripheral blood mononuclear cells(PBMCs) in patients with bronchial asthma and the effects of dexamethasone(DEX) on it. Methods PBMCs were collected from asthmatic patients and normal subjects. Using Western blot and ELISA, the expression of phospho-p38 MAPK in PBMCs and the concentration of IL-4,IL-5 in supernatants were respectively measured. Results The expression of phospho-p38 MAPK in PBMCs and the concentration of IL-4,IL-5 in supernatants were higher in asthmatic group than those in normal group(P<0.01).By DEX treated, the above indexes were decreased significantly compared with those in asthmatic group(P<0.01). There were positive correlations between the expression of phospho-p38 MAPK and the concentration of IL-4,IL-5 in supernatants(r=0.71,0.64,P<0.01).Conclusion p38 MAPK may play a role in pathophysiological process of asthma. DEX could effectively treat asthma by inhibiting the expression of p38 MAPK.