体外受精失败MII期人卵母细胞的免疫荧光研究

Immunofluorescent study of human MII oocytes failed to fertilize after in vitro fertilization

目的探讨体外受精中MII期人卵母细胞受精失败的原因。方法收集体外受精后24～48h仍未受精的MII期卵母细胞,进行免疫荧光染色和碘化丙啶(PI)复染,在荧光显微镜下对其失败原因进行分类。结果卵母细胞内未见精子的在常规体外受精(IVF)周期有55.8%,显著多于卵胞浆内单精子注射(ICSI)周期中的9.7%(P<0.01);卵母细胞活化失败两者分别为14.9%和58.1%,有显著性差异(P<0.01);原核形成和(或)迁移缺陷的在两者分别为25.3%和32.3%(P>0.05);其他异常两者分别为3.9%和0.0%。结论IVF中MII期卵母细胞的受精失败主要是缺乏精子的穿透,ICSI周期中的主要原因是卵母细胞活化不完全。

Objective: The aim of this study was to investigate why some human MIIoocytes failed to fertilize after in vitro fertilization (IVF).Methods: The unfertilized human MII oocytes were collected 24~48 h after IVF or ICSI and stained by FITC-labeled antibody and counterstained with propidium iodide (PI). The types of fertilization failure were identified under the fluorescence microscopy.Results: There were 55.8% unfertilized oocytes found no sperm in them in IVF group, which were more than these in ICSI group (9.7%), (P<0.01). 14.9% unfertilized oocytes in IVF group and 58.1% in ICSI group displayed oocyte activation failure. The difference was significant (P<0.01). Defects in pronuclear formation and/or migration was found in a similar proportion of oocytes in IVF group (25.3%) and ICSI group (32.3%),(P>0.05). Other abnormalities were found in 3.9% oocytes in IVF group but none in ICSI group.Conclusion: The main reason of fertilization failure after IVF was no sperm penetration. However, fertilization failure after ICSI was mainly associated with the incomplete oocyte activation.