期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

小鼠成熟卵丘-卵母细胞复合体蛋白质谱图的建立 被引量：1

Establishment of protein map for mouse mature oocyte-cumulus complex
下载PDF
导出
摘要 目的通过聚丙烯酰胺二维凝胶电泳技术,全面展示小鼠成熟卵丘卵母细胞复合体(COC)的蛋白质表达谱。方法采用13cmpH3～10的非线性胶条进行双向凝胶电泳,分离成熟雌鼠COC总蛋白,并作质谱鉴定。结果获得了较高分辨率的小鼠成熟COC蛋白质表达谱图,并对胶上的3个蛋白点进行了质谱鉴定。结论蛋白质组学是全面分析COC蛋白表达的有效手段。成熟COC蛋白质表达谱的建立为研究卵泡发育机理提供了依据。 Objective: To establish two-dimensional polyacrylamide gel electrophoresis for displaying the full-scale protein expression spectrum of mouse mature oocyte-cumulus complex and evaluate the application of this map in the study of ovarian follicle development.Methods: Mouse oocyte-cumulus complex proteins were separated by two-dimensional gel electrophoresis with 13cm non-linear gradient pH 3~10 strips. The protein spots were identified by mass spectrometry.Results: The high resolution protein map of mouse mature oocyte-cumulus complex was established and three of the spots were identified by mass spectrometry.Conclusion: Proteomics offers an efficient means for a comprehensive analysis of oocyte-cumulus complex protein expression. The protein map of oocyte-cumulus complex will provide a basis for the research of folliculogenesis and the further proteomics studies of oocyte-cumulus complex.
作者 孟艳 马翔 钱云 沙家豪 刘嘉茵 王淑玉
机构地区 南京医科大学第一附属医院妇产科临床生殖中心 南京医科大学生殖医学实验室
出处 《生殖医学杂志》 CAS 2005年第2期98-103,共6页 Journal of Reproductive Medicine
基金 江苏省科学技术厅社会发展基金(BS2003045) 高校省级重点实验室开发课题(KJS02042) 江苏省卫生厅135生殖医学重点学科基金
关键词 双向凝胶电泳 质谱技术 卵丘-卵母细胞复合体 蛋白质谱图 Two-dimensional electrophoresis Mass spectrometry Oocyte-cumulus complex Protein map
分类号 R711.6 [医药卫生—妇产科学]
  • 相关文献

参考文献16

  • 1Yates JR 3rd, Speicher S, Griffin PR, et al. Peptide mass maps: a highly informative approach to protein identification[J]. Anal Biochem, 1993, 214 (2) : 397-408.
  • 2Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J]. Anal Biochem, 1976,72 : 248-254.
  • 3Gorg A , Postel W , Weser J , et al. Elimination of point straking on silver-stained two-dimensional gels by addition of iodoacetamide to the equilibration buffer[J]. Electrophoresis, 1987, 8: 122-124.
  • 4Berkelman T, Stenstedt T. 2-D electrophresis using immobilized pH gradients: principles & methods[M]. Sweden: Amersham Pharmacia Biotech Inc, 1998. 36.
  • 5Neuhoff V , Arold N , Taube D, et al. Improved staining of proteins in polyacrylamide gels including isoelectric focusing gels with clear background at nanogram sensitivity using coomassie brilliant blue G-250 and R-250[J]. Electrophoresis,1988, 9 (6) :255-262.
  • 6Shevchenko A , Wilm M , Vorm O,et al. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels[J]. Anal Chem, 1996, 68 (5) : 850-858.
  • 7Matzuk MM, Burns KH, Viveiros MM, et al. Intercellular communication in the mammalian ovary: oocytes carry the conversation[J]. Science,2002,296(5576):2178-2180.
  • 8Sirover MA. New insights into an old protein: the functional diversity of mammalian glyceraldehyde-3-phosphate dehydrogenase[J]. Biochim Biophys Acta, 1999,1432(2) : 159-184.
  • 9Kusner LL, Sarthy VP, Mohr S. Nuclear translocation of glyceraldehyde-3-phosphate dehydrogenase., a role in high glucose-induced apoptosis in retinal Muller cells[J]. Invest Ophthalmol Vis Sci, 2004,45 (5) : 1553-1561.
  • 10Mazzola JL, Sirover MA. Alteration of intracellular structure and function of glyceraldehyde-3-phosphate dehydrogenase: a common phenotype of neurodegenerative disorders[J]? Neurotoxicology, 2002,23 (4-5) : 603-609.

同被引文献14

  • 1Blackstock WP,Weri WP.Proteomics:quantitative and physical mapping of cellular proteins[J].Trends Biotechnol,1999,17(3):121-127
  • 2Anderson NL,Anderson NG.Proteome and proteomics:new technolo2 gies,new concepts,and new words[J].Electrophoresis,1998,19(11):1853-1861.
  • 3Coonrod SA,Wright PW,Herr JC.Oolemmal proteomics[J].J Reprod Immunol,2002,53(1-2):55-65.
  • 4Tanaka M,Nakajin S,Kobayashi D,et al.Teleost ovarian carbonyl reductase – like 20 beta – hydroxsteroid dehydrogenase:potential role in the production of maturation – inducing hormone during final oocyte maturation[J].Biol Reprod,2002,66(5):1498-1504.
  • 5Anahory,T,Dechaud H,Bennes R,et al.Identification of new proteins in follicular fluid of mature human follicles[J].Electrophoresis,2002,23(7-8):1197-1202.
  • 6Byrjalsen I,Larsen PM,Fey SJ,et al.Two2dimensional gel analysis of human endometrial protein:cyclic changes in the expression of specif2 ic proteins during the normal menstrual cycle[J].Hum Reprod,1995,10 (1):13218.
  • 7Byrjalsen I,Larsen PM,Fey SJ,et al.Human endometrial proteins with cyclic changes in the expression during the normal menstrual cy2cle:characterization by protein sequence analysis[J].Hum Reprod,1995,10 (10):2760-2766.
  • 8Fouchecourt S,Lareyre JJ,Chaurand P,et al.Identification,immunolocalization,regulation,and postnatal development of the lipocalin EP17(epididymal protein of 17 kilodaltons) in the mouse and rat epididymis[J].Endocrinology,2003,144(3):887-900.
  • 9Wolkowicz MJ,Naaby-Hansen S,Camble AR,et al.Tektin B1 demonstrates flagellat localization in human sperm[J].Biol Reprod,2002,66(1):241-250.
  • 10Ursini F,Heim S,Kiess M,et al.Dual function of the selenoprotein PHGPx during sperm maturation[J].Science,1999,285(5432):1393-1396.

引证文献1

生殖医学杂志
2005年 第2期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部