太空诱变玉米细胞核雄性不育基因与RAPD标记的连锁分析

Screening a RAPD Marker Related to the Maize Male Sterility Gene Obtained by Space Flight

以姊妹交多代的太空诱变玉米雄性不育材料RP3195(A)×S37(自交系)得到的F2代分离群体(138株)为材料,利用集团分离法(BSA)对太空诱变玉米细胞核雄性不育基因进行了RAPD分析,在152个随机引物中,引物SB SK-14(CCCGCTACAC)和SBSR-3(ACACAGAGGG)分别在可育集团与不育集团之间扩增出多态性产物SBSK-14850、SBSK-14475和SBSR-3400。通过对F2代分离群体中的单株进行连锁分析,初步认为SBSK-14850、SBSK-14475和SBSR-3400与太空诱变玉米细胞核雄性不育基因相连锁,遗传距离分别为49.6,26.6和31.7cM。

F_2 segregation populations derived from maize sterility materials RP^3195 (A)×S_(37) (inbred line), which had been sib-bred for many generations, was used as experiment material in this research. Bulked segregation analysis (BSA) strategy was employed to identify random amplified polymorphic DNA (RAPD) marker linked to the maize male sterility gene. A total of 152 arbitrary 10-mer oligonucleotide primers were screened on the DNA of fertile and sterile bulks. Primer SBSK-14 (CCCGCTACAC) and SBSR-3 (ACACAGAGGG) give repeatable polymorphism between the two bulks. Through the linkage analyzing of the single plant of F_2 segregation populations, the results indicate that the RAPD makers, SBSK-14_(850), SBSK-14_(475) and SBSR-3_(400) are linked to the maize male sterility gene, and have a map distance of 49.6, 26.6 and 31.7?cM respectively.