小鼠胎儿成纤维细胞饲养层制备的研究

Study on preparation of the MEF feeder layer

研究主要探讨在制备小鼠胎儿成纤维饲养层中,不同处理条件对小鼠胎儿成纤维细胞分裂与存活的影响.结果表明:小鼠胎儿成纤维细胞用适当处理浓度的丝裂霉素-C或适当强度的γ-射线处理一定的时间(10 μg/mL 1～4 h,20 μg/mL 1～2.5 h或21 Gy和28 Gy各处理1 h),能有效地抑制其分裂,且不影响其活力.

Carring the study is to investigate the influence on split and survival of the MEF in different condition . The result : mytomycin-c (10μg/mL 1～4 h,20 μg/mL 1～2.5 h) or γ-rays(21 Gy 1 h,28 Gy 1h) can restrain the abruption of the MEF effectively, but don′t affect the vigor .