原位杂交法检测MDR1基因在食管癌中的表达及与p53的关系

Expression of MDR1mRNA detected and its correlation with p53 in esophageal carcinoma by in situ hybridization

目的:了解食管癌组织MDR1基因表达的临床意义及其与p53的关系。方法:用原位杂交法检测了57例食管癌组织标本中MDR1基因的表达,免疫组化法检测了其中44例p53蛋白的表达并进行临床病理分析及相关性分析。结果:57例食管癌组织中MDR1mRNA阳性表达率为35%(20/57),其中食管癌无淋巴结转移的患者阳性表达为29%(8/29),有淋巴结转移患者阳性表达分别为41%(12/28),高于无淋巴结转移患者的表达。临床Ⅱ期食管癌患者阳性表达分别为27%(8/30),临床Ⅲ-Ⅳ期阳性表达为44%(12/27),高于临床Ⅱ期患者的表达。食管癌患者MDR1mRNA表达与肿瘤大小、部位、分化程度、肿瘤浸润的程度无明显相关。44例食管癌组织中p53蛋白阳性表达率为73%(32/44),其中食管癌浸润至肌层,p53表达率为45%(5/11),肿瘤浸润至外膜,p53表达率为82%(27/33),差异有显著性,P<0.01。食管癌患者p53表达与肿瘤大小、部位、分化程度、有无淋巴结转移、临床分期无明显相关。32例p53蛋白表达阳性的组织中有10例MDR1mRNA表达阳性,它们之间的表达在统计学上无明显相关。结论:MDR1基因及突变型p53在食管癌组织中均有过表示,其表达可能与食管癌进展相关。在食管癌组织中MDR1基因表达可能并非受p53的调控。

Objective To explore the clinical significance of multidrug resistance (MDR1) expression and its correlation with P 53 in esophageal carcinoma.Methods The expression of MDR1 by in situ hybridization in 57 esophageal carcinomas and the expression of P 53 by immunohistochemistry in 44 of 57 esophageal carcinomas were analyzed,and then evaluated their relationship with clinicopathological factors and their correlation.Results The positive expression rate of MDR1mRNA was 35%in 57 esophageal carcinomas.Eight positive expression cases (29%) were found in 28 cases without lymph node metastasis,and 12 positive expression cases (41%) were found in 29 cases with lymph node metastasis;Eight positive expression cases (27%) were found in 30 clinical II stage cases,and 12 positive expression cases (44%) were found in 27 clinical III-IV stage cases.No significant correlation was found among MDR1mRNA expression and tumor size,position,differentiation degree and infiltration degree.The positive expression rate of mutation P 53 was 73%in 44 esophageal carcinomas.Five positive expression cases (45%) were found in 11 cases that tumor cells invaded to muscle,and 27 positive expression cases (41%) were found in 33 cases that tumor cells invaded to serous membrane.Significant difference between them was found(P< 0.01).No significant correlation was found among mutation P 53 expression and tumor size,position,differentiation degree,lymph node metastasis state and clinical stage.Ten MDR1mRNA positive expression cases were found in 32 P 53 positive expression cases,no significant correlation between them(P< 0.01).Conclusion Over expressions of MDR1mRNA and mutation P 53 were significantly associated with esophageal carcinoma progression.The expression of MDR1mRNA might not be regulated by mutation P 53.