CD80与CD86在角膜移植排斥反应中作用的实验研究

Experimental study of CD80 and CD86 in the immunity rejection of rat corneal transplantation

目的:探讨角膜移植术后C D80、C D86的表达,了解协同刺激分子在角膜移植排斥反应中的作用,为进一步研究角膜移植排斥反应发病机制及治疗提供新的思路。方法:随机将F344大鼠5只及Lou大鼠25只分为同种异体移植组和同基因移植对照组,同种异体组采用F344大鼠5只为供体,Lou大鼠10只为受体,对照组采用Lou大鼠5只作为供体和Lou大鼠10只作为受体。连续观察12d,进行临床及病理学观察。采用流式细胞技术检测角膜细胞在移植术后C D80、C D86的表达。结果:同种异型移植组术后角膜细胞C D80、C D86的表达较对照组高,差异有显著性(P<0.01),并且与临床及病理学观察结果相一致。结论:协同刺激分子C D80、C D86参与了角膜移植排斥反应,角膜移植术后植片协同刺激分子表达增加从而活化局部T细胞的功能,使免疫排斥反应发生。

Objective To investigate the role of CD80 and CD86 in the immunity rejection of rat corneal transplantation. Methods The rat orthotopical corneal transplantation model was developed. Five F344 rats and 25 Lou rats were randomly divided into two groups:Syngeneic control(Lou to Lou) and allograft group(F344 to Lou).Flow cytometry was used to analyze CD80 and CD86 expression of corneal graft in each group. Results The expression of CD80 and CD86 on the allotype group corneal cells after corneal transplantation was higher than that of control group. The difference was significant(P< 0.01). And the expression level of CD80 and CD86 had nicely correlated with rejection index(RI). Conclusion Costimulatory molecule plays a critical role in the corneal graft rejection. The increasing expression of costimulatory molecule on corneal cells after corneal transplantation activates local T cells and initiate the corneal graft rejection.