蚓激酶基因克隆及序列分析

Cloning and Sequencing of Lumbrokinase Gene

目的:克隆蚓激酶基因并在GENEBANK中进行序列分析 方法:采用RT -PCR技术,以蚯蚓总RNA为模板进行扩增,克隆蚓激酶基因 用Blast软件对基因进行同源性分析 结果:克隆了一个cDNA片段,与GENEBANK中蚓激酶基因序列最高同源性为99% 结论:本方法实用可行,同源性分析表明克隆的cDNA片段具备完整的蚓激酶编码区。

Objective: To clone lumbrokinase gene and undertake sequence analyze in GENEBANK. Methods: Adopting RT-PCR techniques to amplify lumbrokinase gene using the total RNA of earthworm as template. Results: cDNA fragment were amplified and then cloned. After sequencing and analyzing the sequences by Blast, we find the highest sequence similarity was up to 99% between the sequence we submitted and that of lumbrokinase gene exited in GENEBANK. Conclusion: The method is feasible and the sequence analyzing have demonstrated that the two cDNA fragment have the whole coding sequence of lumbrokinase, which laid a good foundation for the study of the expression of lumbrokinase.