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骨髓干细胞移植治疗心肌梗死最佳时间段的选择 被引量:13

Optimal Time for Bone Marrow Cells Transplantation to Maximize Heart Function after Myocardial Infarction
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摘要 目的:讨论心肌梗死后经冠脉骨髓单个核细胞移植的最佳时间段.方法:建立大鼠心肌梗死模型,观察心肌梗死后1d、1周、2周及4周心脏的组织学改变,并且分别在心肌梗死后1d(n=10)、1周(n=10)、2周(n=10)及4周(n=10)非选择性经冠脉内移植骨髓单个核细胞或PBS,4周后观察梗死面积大小及瘢痕厚度.超声心动图观察移植前后心功能和重构指标变化.结果:心肌梗死后1d炎症反应最严重,4周时瘢痕扩展并变薄(P<0.05).心肌梗死后1周及2周,细胞移植组梗死面积缩小,胶原厚度增加最明显(P<0.05);2周组心功能改善最明显(P<0.05);1周组其次(P<0.05).结论:心肌梗死后1~2周进行骨髓干细胞移植得益最大. Objective: To determine the optimal time for bone marrow mononuclear cells (MNCs) transplantation (BMT) after myocardial infarction (MI). Methods: MI was induced in inbred rats by left anterior descending artery ligation. Histological studies was examined in infarcted hearts at 1d, 1, 2, and 4weeks post MI (n=3 each), and bone marrow mononuclear cells (BM-MNCs) were collected from these rats bone marrow. A total of 1×10 7 MNCs or Phosphate Buffer Solution (PBS) were administered through coronary artery at above mentioned time points accordingly (n=10 each). 4 weeks later, infarct size and scar thickness were evaluated by histology. Cardiac function was assessed by echocardiography pre-transplantation or 1 month post-transplantation. Results: The inflammatory reaction was greatest 1 day post-MI. Scar size expanded and thinned 4 weeks post-MI(P<0.05). 1 month post-transplantation, infarct size was smaller and collagen thickness was greater in the rat hearts 1 week and 2 weeks after transplantation than that in the other groups (P<0.05). Cardiac function enhancement and remodeling improvement were more significant in rats received intracoronary BM-MNCs injection within 1-2 weeks post MI than that those at other time-points.Conclusion:1-2 weeks post MI might be the best time point for intracoronary bone marrow cell transplantation, which might more benefit for cardiac function recovery and remodeling improvement.
出处 《中国临床医学》 北大核心 2005年第2期192-195,共4页 Chinese Journal of Clinical Medicine
基金 "十五"国家科技攻关计划项目(2004BA714B05 2) 上海市科委优秀学科带头人计划项目(03XD14010)资助
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二级参考文献42

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