利用大孔吸附树脂分离纯化葛根素

The separation and purification of puerarin by Macro reticular resin

为了更好地分离纯化葛根素,选取 6种大孔吸附树脂进行了筛选实验,同时采用了紫外分光光度法和HPLC进行了研究。结果表明,由紫外数据显示,AB-8树脂更适合葛根素的分离纯化;但HPLC测定表明,AB-8在吸附葛根素(208mg·g-1 )的同时,也吸附了较多的杂质-4’-甲氧基葛根素(11 6%)。因而采用了吸附量相对较小的D101(182mg·g-1 )。最终建立了一条大孔吸附树脂初步分离结合醋酸结晶的方法分离纯化葛根素。分离结果显示葛根素纯度≥97%。该方法操作简便,成本低,收得率高,适于工业化生产。

In order to separate and purify puerarin from Pueraria lobata (Willd), six different Macro reticular resins had been used with technology of both UV and HPLC . The results from UV show that the resin of AB-8 is better to separate and purify puerarin.But the results from HPLC also show that AB-8 not only absorb puerarin (208mg·g^(-1)) , but also absorb much impurity-4’-methyloxidie puerarin(11.6%). So we select the D101 which has a smaller absorption (182mg·g^(-1)).And after running through the column of D101, the amounts of puerarin and impurity are 89% and 5.9%, and in contrast with AB-8, the results are 71% and 9%, which means that D101 is fitter for our experiments.Finally, the method of separation by Macro reticular resin and then crystallization by acetic acid has developed, which produces the purification of puerarin no less than 97%.This method is easily performed, has low cost while the final purification is high and fit for industrial production.