糖尿病患者血尿转化生长因子β1变化及其意义

The change of serum and urinary transforming growth factor-beta-1 and their clinical significances in type 2 diabetics

目的 观察2型糖尿病患者血、尿转化生长因子-β1 (TGF -β1 )变化,探讨TGF- β1与糖尿病肾病关系及其检测的临床意义。方法 对79例2型糖尿病患者和2 0例健康人,采用双抗体夹心酶联免疫吸附试验(ELASIA)同时检测血和尿中TGF β1浓度。结果 与正常对照比较,糖尿病患者血TGF β1 ( 4 5 .57±2 1 . 78vs 2 4 . 58±1 2 .6 1ng/ml)、尿TGF- β1 ( 6 1 . 6 8±35 .92vs 1 9 .89±1 3 0 6ng/mmol Cr)均增高,差异有显著意义,随肾病进展血、尿TGF- β1增高愈明显。在正常、微量和大量白蛋白尿糖尿病患者之间,血、尿TGF- β1水平存在显著差异;血TGF -β1、尿TGF -β1与2 4h尿白蛋白排泄率呈正相关(r =0 . 397,P <0 . 0 5;r =0 . 537,P <0 .0 5)。结论 糖尿病患者血、尿TGF -β1明显升高,并与糖尿病肾病进展一致。血、尿TGF- β1可以作为糖尿病肾病敏感的早期诊断指标之一。

Objective To observe the changes of serum and urinary transforming growth factor beta-1(TGF-β1) in type 2 diabetes and explore the clinical value of TGF-β1 in diabetic nephropathy. Methods Using enzyme-linked immunoadsorbent assay(ELISA), serum and urinary TGF-β1 levels were detected in 79 patients with type 2 diabetes and 20 controls. Results Serum and urinary TGF-β1 levels increased significantly in diabetic patients compared with the controls, and further rose as the nephropathy progressed. When compared with each other, there were significant differences in serum and urinary TGF-β1 levels between normol, micro-and macro-albuminuria diabetic groups( P <0.05). Serum and urinary TGF-β1 levels were positively correlated with urinary albumin excretion. Conclusion TGF-β1 may play an important role in pathogenesis and development of diabetic nephropathy. Serum and urinary TGF-β1 measurement may be one of the early markers in the diagnosis of diabetic nephropathy.