应用膜分离技术改进林可霉素提炼工艺

Membrane separation technique applied to improving extraction of lincomycin

介绍了在混合醇萃取前, 引入超滤纳滤组合膜分离技术对板框过滤后的林可霉素发酵液进行提纯和浓缩的小试结果: 碱化料液的膜分离效果优于未碱化料液; 对不同材料(PES、PS、PA) 和相对截留分子质量的超滤和纳滤膜的筛选结果表明, PES -10 和NF- 270 膜在膜通量和林可霉素收率、杂质去除等方面优于其他的膜,为最佳选择; 超滤纳滤后再进行萃取, 不但混合醇的用量因料液体积减小而相应减少, 并且萃取效率和产品收率都得到提高, 当纳滤浓缩倍数为1 28 时, 一次萃取率和总收率分别比原来无超滤纳滤时增加了4. 3%和3 .4%, 所得萃取液的色度也较低.

Separation processes with ultrafiltration and nanofiltration were introduced to purify and concentrate the fiber-board filtered lincomycin broth in front of mixed-alcohol extraction. Bench scale test indicated that the application to the alkalized feed was superior to the non-alkalized feed. After comparing several membranes with different materials (PES, PS, PA) and molecular weight cutoffs, PES-10 and NF-270 were found to be the optimum UF and NF membranes. Successive extraction after UF-NF needed less extractant due to the reduced feed volume. Both the extraction efficiency and product yield were improved. For NF concentration multiple of 1.28, the mixed-alcohol once-extraction rate and the general product yield increased by 4.3% and 3.4% respectively. The color of extract phase was lighter than the ones without UF-NF.