传染性非典型肺炎患者血清蛋白质指纹图谱分析

Analysis of protein fingerprint spectrum in sera from patients with severe acute respiratory syndrome

目的探索表面增强激光解析离子化飞行时间质谱技术(SELDI)在传染性非典型肺炎又称[严重急性呼吸综合征(SARS)]早期诊断中的应用价值。方法用SELDI检测SARS患者组和对照组血清,建立诊断模型,然后进行单盲模型验证。结果通过检测37例SARS患者和74名健康对照者血清,发现早期SARS患者血清中有4种特异性蛋白质荷比峰(M/Z)-3939.08、4137.71、8136.64和11514.20,其中4137.71下调,另3种上调,并以此建立SARS诊断模型,对37例SARS患者和73名对照者血清进行单盲检测,敏感性为97.3%(36/37),特异性为91.8%(67/73)。结论用SELDI可从早期SARS患者中筛选出4个特异蛋白质荷比峰,以此建立的诊断模型可配合临床对SARS患者进行早期诊治。

Objective To discover the applying value of surface-enhanced laser desorption/ionization time-of-flight mass spectrometry technology in early diagnosis of severe acute respiratory syndrome.Method The serum samples of acute SARS group and control group were detected by SELDI technology, first established diagnosis model, then validated model single-blindly.Result Through detection of acute SARS group (37 cases) and control group (74 persons), four specific protein fingerprint biomarkers, which masses were at 3 939.08、4 137.71、8 136.64、11 514.20 were found. Except the second protein down-regulated, the others was up-regulated in acute SARS patients. Based on the results, a diagnosis model was established. In order to validate the model, the serum samples of SARS patients and controls were detected and permitted a sensitivity of 97.3% and specificity of 91.8% for the study population.Conclusions Acute SARS patients have specific protein fingerprint spectrum in sera. Detecting the serum samples of SARS patients or suspected patients by SELDI technology can aid the early diagnosis better in clinics.