芦荟愈伤组织诱导的初步研究

A Preliminary Study on Callus Induction of Aloe barbadensis

以库拉索芦荟的茎段、茎尖、叶片和根为外植体,以MS为基本培养基,添加不同浓度的2,4-D,NAA,KT或其组合,并附加AgNO3,Vc,研究外植体种类、大小,接种方式,激素配比,以及AgNO3,Vc对库拉索芦荟愈伤组织诱导及生长情况的影响.结果表明,外植体以2cm长度的茎段较好;接种方式以横放效果较好;NAA15mg/L+2,4-D4mg/L+KT0.1mg/L的激素组合愈伤组织诱导率达到90%;添加AgNO3诱导出的愈伤组织生长旺盛,富有光泽,以10mg/L效果较好;添加Vc对愈伤组织的褐化有一定的抑制,以5mg/L效果较好.

The influence upon callus induction and its growth by the category and size of the explants, method of implanting, combination of plant growth regulators, and the effect of AgNO_3 and vitamin C was studied when the segments of stem, shoot, leaf and root of Aloe barbadensis were applied as explants, MS was adopted as elementary medium added with varied quantities of 2,4-D, NAA, KT and the different combinations of these plant growth regulators, and different contents of AgNO_3 and Vitamin C were added into the medium. It was indicated by the results that the best explants were 2 cm long stems, the best implanting method was the horizontal placement, the best combination of plant growth regulators was NAA 15 mg/L +2,4-D 4 mg/L +KT 0.1 mg/L, with which the callus induction rate reached to be 90%. It was also showed that the addition of 10 mg/L AgNO_3 could promote the growth of the callus, and 5 mg/L of vitamin C was helpful to inhibit callus browning in the culture process.