摘要
目的:测定匝迪-5中胡椒碱的含量.方法:采用HPLC法.结果:进样在0.24~1.2μg有良好的线性关系,r=0.9998,平均回收率为98.1%,CV=1.92%(n=5).结论:该方法分离效果好,简便、快速,结果准确,使用于测定匝迪-5中胡椒碱的含量.
Objective:To determine the content of piperine in the Mongolian medicine Zadi-5 pills.Methods:The content of piperine in Zadi-5 was determined by RP-HPLC.The mobile phase was methyl-water(V∶V=74∶26) with a flow rate of 0.8 ml·min^(-1),the wavelength of the detector was set at 343 nm.Results:The linear range was 0.24~1.2 μg,and correlation coefficient was 0.9998.The average recovery and relative deviation were 98.1% and 1.9% respectively.Conclusion:The method is simple,accurate,and could be used for the determination of content of piperine of Zadi-5 pills.
出处
《内蒙古民族大学学报(自然科学版)》
2005年第2期213-214,共2页
Journal of Inner Mongolia Minzu University:Natural Sciences