新生儿肠闭锁NT-3与Trk-C表达研究

Expression of NT-3 and Trk-C protein in congenital intestinal atresia of newborn.

目的 了解神经节细胞NT - 3、Trk -C、Nf蛋白和神经胶质细胞GFAP蛋白在肠闭锁中的分布情况,并探讨其临床意义。方法 应用免疫组化技术检测15例先天性小肠闭锁患儿肠壁内NT - 3、Trk -C、GFAP和Nf的表达情况,并以死于与肠道发育异常无关的尸检新生儿小肠标本6例作为正常对照。结果 肠闭锁组肌间神经丛NT - 3阳性细胞数明显低于对照组的阳性细胞数(t′=9.90 0 0 ,P <0 .0 5 )。Trk -C在对照组肠壁肌间神经丛中存在高表达,而在肠闭锁组存在低表达(t=2 2 .2 991,P <0 .0 5 )。对照组肠壁肌间神经丛胶质细胞GFAP呈阳性表达,而肠闭锁组GFAP呈强阳性表达。Nf在对照组中的表达明显高于闭锁组。结论 先天性小肠闭锁的近端肠壁内存在有神经节细胞的减少和神经干的变细,支持细胞在闭锁近端肠壁中呈增生表现。这种肠壁肌层神经丛的改变,可能会引起肠腔内压力减低,从而影响闭锁区域肠道的功能。

Objective: This study aims to evaluate the distribution of ganglia (NT-3, Trk-C, Nf) and enteric glial cells (GFAP) in congenital intestinal atresia and discuss the clinical significance. Methods: Immunohistochemical analysis of small bowel from patients with congenital intestinal atresia(n=15) and normal intestine(n=6) was performed for NT-3, Trk-C, GFAP and Nf. Results: A significantly higher proportion of myenteric plexuses neurons were NT-3 in control than small bowel atresia ( t′=9.9000,P <0.05). A significantly higher proportion of myenteric plexuses were Trk-C ( t=22.2991,P <0.05) and Nf in control than small bowel atresia. The proportion of myenteric plexus glial cells in the intestinal atresia expressed higher GFAP than control. Conclusion: There is a near total of ganglia and axonal network in myenteric plexus of congenital intestinal atresia and proliferative glial. Our results suggested that the change of myenteric plexuses in congenital intestinal atresia might be reduced pressure of intestinal volume and affected function of atretic intesine.