1572份广东汉族人群脐血HLA分布及连锁不平衡分析

Distribution and linkage disequilibrium at HLA of Guangdong Han people in Guangzhou cord blood bank.

目的 总结广州脐血库中HLA分型资料,分析广东省汉族人群HLA分布特点。方法 采用单克隆板和寡核苷酸探针杂交方法对15 72份脐血检测HLA -A、B、DRB1抗原,用PCR -SSP及SBT方法对有疑问的结果进行复核。结果 共检出HLA -A抗原18种,B抗原4 0种,DRB1抗原13种,有显著统计意义的HLA -A -B单倍型有70种,扩展型单倍型(HLA -A -B -DRB1) 2 0 0种。结论 广东省汉族人群HLA分布有自身特点,与北方汉族等人群有着显著差异,与台湾人群HLA分布较为接近。

Objective: To summarize in the Guangzhou cord blood bank HLA minute material, analyze the Guangdong Province Han Nationality HLA distributed characteristic. Methods: Use MLT(micro lymphocytetotoxicity test) and the SSOPH method detect HLA-A, B, and DRB1 antigen to 1,572 cord blood, some samples has question with PCR-SSP and the SBT method to redetect. Results: Altogether picks out the HLA-A antigen 18 kinds, the B antigen 40 kinds, the DRB1 antigen 13 kinds. The remarkable statistical significance HLA-A-B Linkage Disequilibrium 70 kinds, (HLA-A-B-DRB1) 200 kinds. Conclusion: Guangdong Province Han Nationality HLA distribution has own characteristic, with the north Han Nationality has the remarkable difference, approaches with the Taiwan HLA distribution.