柑橘溃疡病菌PCR快速检测技术研究初报

Rapid PCR detection of citrus bacterial canker disease

应用柑橘溃疡病菌独有的保守基因设计并合成了引物对,建立柑橘溃疡病菌PCR检测技术。PCR检测结果 表明,来源于不同产地的病菌菌株均可扩增出靶标带,而水稻白叶枯病菌不能扩增出靶标带;2×10～2×105 个·ml-1的溃疡病病菌悬浮液也均可扩增出靶标带,说明该PCR技术具有很强的特异性和检测灵敏度。研究结果也 表明,来源于福建、四川和安徽的柑橘溃疡病菌具有同源性。

The samples of the citrus bacterial canker disease (Xanthomonas campestris) collected from Fujian. Sichuan and Anhui in China during 2002-2003 were determined by PCR using one pair of specific primers (P1/ P2) designed based on published sequence. PCR amplification product of 493 bp target band was generated from target pathogen of X. campestris but not from non-target pathogenic bacteria of X.oryzae. Resucts showed that there was a remarkable homogeneity among the pathogens of diseased citrus from Fujian, Sichuan and Anhui, and there were specificity and sensitivity to detecting pathogen DNA of X. campestris by using PCR.