双波长薄层扫描法测定产后康口服液中盐酸水苏碱的含量

Determination of Stachydrine Hydrochloride in Chanhoukang Oral liquid By Dual Wavelength TLC Scanning

目的 建立产后康口服液中盐酸水苏碱含量测定的方法。方法 选用的展开剂为丙酮-无水乙醇-盐酸(10∶10∶1) ;测定波长:λS=5 10nm ,λR=70 0nm ;用双波长薄层反射锯齿扫描;狭缝:0 .4mm×0 .4mm ;线性参数:SX =7,灵敏度:X1;扫描速度:12mm/min。结果 盐酸水苏碱回归方程为:A =96 6 6 .0 6 +112 4 95 .3C ,r =0 .9992 ,平均回收率为98.3% ,RSD为1.2 0 %(n =6 ) ;在1.0～5 .0 μg范围内线性关系良好。结论 本法操作简易、准确、重复性好。

Aim To establish a method using dual wavelength TLC- Sc anning for the determination of Stachydrine in Chanhoukang Oral liquid. Methods Developer was CH 3COCH 3-anhydrous C 2H 5OH-HCl(10∶10∶1). The determination was carried out by the reflection zigzag scanning,SX=7,slit= 0.4mm×0.4mm,sensitivity:X1,scan speed:12mm/min,and wavelength was λ S =5 10nm,λ R =700nm.Results The mean recovery was 98.3%,RSD was 1.20% in the Linearity range of 1.0～5.0μg,(r=0.999 2).Conc lution It was proved that the method was simple,accurate and reproducibl e for the determination of Stachydrine in Chanhoukang Oral liqiud.