应用大鼠肝细胞原代培养作为短期致突变测试体外介导活化系统的研究

Study on the Application of Cultured Rat Hepatocyte-mediation as Activation System for Short-term Mutagenicity Test

本研究建立了以原代培养的大鼠肝细胞作介导细胞、以中国地鼠肺V79细胞和鼠伤寒沙门氏菌组氨酸缺陷型突变株(TA100)为靶细胞的检测系统。显示前致癌物黄曲霉素B_1经肝细胞介导后致突变效应显著加强,从而证实了体外肝细胞介导活化的有效性,并讨论了在体外致突变试验中利用肝细胞作活化系统的有关问题。

A detective system was established in wtlich rat hepatocyte primary culture serves as mediated cell, Chinese hamster cell lines ( V(?)) and Salmonella typhimuriun mutation strain TA100 is the target bacteriaIt showed that the mutangenic-effect of pre-carcinogen-aflatoxin B1 has been increased great ly by hepatocyte mediation. It is proved that the activation of hepatocyte mediated in vitro is effective.