利用区带毛细管电泳分离、分析葡萄籽原花青素

Analysis of proanthocyanidin of grape seed by capillary electrophoresis

通过选择不同的缓冲液,发现硼酸盐缓冲液对含有大量同分异构体和不同聚合度的葡萄籽原花青素分离、分析具有良好的效果,利用毛细管电泳可以将葡萄籽原花青素各组分进行一定程度的分离,得到较好的电泳图谱。当硼砂-磷酸盐缓冲液pH=9,浓度为80mmol/L时,原花青素混合物的分离度和分离效果最好。提高毛细管电泳分离电压和分离温度,降低硼砂-磷酸盐缓冲液的浓度,可以改善各组分的分离效果,缩短分离时间,提高分析速度。通过解聚反应,可使高聚体原花青素降解,葡萄籽原花青素的电泳分离效果得到明显改善,在低浓度(20mmol/L)硼砂-磷酸盐缓冲液中也可得到艮好的毛细管电泳图谱。

Choosing different buffer solution, we find that the borate buffer has better effects than others on the analysis of proanthocyanidin with different isomers in the capillary electrophoresis. When the pH of borate buffer is 9, and the concentration is 80mmol/L, the components of proanthocyanidin could be separated very well, and a good chromatography of capillary electrophoresis could be obtained. Increasing the voltage and temperature of capillary electrophoresis and decreasing the concentration of borate buffer, the separating time of proanthocyanidin can be shortened; the analytic speed of proanthocyanidin can be improved. Depolymerizing proanthocyanidin in the presence of excess phloroglucinol under acidic conditions, the cleavage products of proanthocyanidin become easy to analysis in the capillary electrophoresis, and a good chromatography of proanthocyanidin was observed in the low concentration of 20mmol/L borate buffer.