杀虫畏对雄性大鼠肝微粒体酶的诱导及动力学性质的研究

Effects of Tetrachlorvinphas on Hepatic Microsomal Enzymes and Its Kinetic properties

杀虫畏对肝微粒体酶呈现明显诱导作用。对诱导生成的酶的动力学性质进行研究证明,诱导生成组和对照组的苯胺羟化酶,其米氏常数(km)和抑制常数(ki)极相近似;微粒体酶抑制剂SKF525-A对两组酶均呈现非竞争性抑制作用。因此,可以认为两组酶具有相同的动力学性质。

Tetrachlorvinphas(TCVP)was admnistratid to the rats at 150mg/kg po. for 7 days The resuIts showed that TCVP May induce apparontly the hepatc microsomal enzymcs, The kinetic .properties qf control and induced aniline hydroxylase were also studied. It was observed that Michaelis constant ( km = 1.098 ±0.175 ) is similar. Inhibitor constant ( ki=2.882±0.159 ) is also simil ar. Furthermore, both enzymes were inhibited noncompetitively by irhibitor SKF525A.