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对HCMVUL54 mRNA片段特异性切割的M1GS构建 被引量：4

Construction of M1GS for Targeted Cleavage of HCMV UL54 mRNA Segments
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摘要 人巨细胞病毒是一种DNA病毒,在人群中一般呈亚临床感染和潜伏感染。为研究病毒基因沉默工具和抗病毒制剂,以人巨细胞病毒UL54基因mRNA序列设计互补的外部引导序列,共价结合到大肠杆菌来源RNaseP催化核心M1RNA上,从而构建成M1GS-T6核酶。通过对DNA聚合酶UL54基因亚克隆片段转录产物体外切割研究,证实该核酶具备对UL54mRNA片段的特异切割能力。 Human cytomegalovirus (HCMV) is a DNA virus and serious opportunistic pathogen for both newborn and immunocompromised individuals.To research technique for gene silence and antiviral agents, ribozyme M1GS-T6 was constructed from external guide sequences(EGSs)that consist of a sequence complementary to HCMV UL54 gene RNA and M1 RNA, the catalytic RNA subunit of RNase P from Escherichia coli. The results showed that M1GS can efficiently cleave the mRNA sequence encoding UL54 protein in vitro.
作者 吕静竹 李弘剑 陈浩军 李月琴 周天鸿 唐冬生 张欣
机构地区 暨南大学生命科学技术学院
出处 《微生物学通报》 CAS CSCD 北大核心 2005年第2期83-86,共4页 Microbiology China
基金 国家自然科学基金资助项目(No.30370776) 广东省自然科学基金重大项目(No.36703) 广东省自然科学基金(No.021162 000718)
关键词 人巨细胞病毒 UL54基因 RNASE P 外部引导序列 Human cytomegalovirus(HCMV),UL54,RNase P,External guide sequences (EGSs)
分类号 R346 [医药卫生—基础医学]
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参考文献10

  • 1Phong T, Manfred L , Edward N , et al. PNAS , 2000 ,97 (11) : 5812～5817.
  • 2Walter D , Phong T, Umair K, et al. Proc Natl A1cad Sci USA, 2001 , 98 (26): 14831～14836.
  • 3Markus W, Detlef M, Peter S, et al. J of Virology, 2000 , 74 (22) : 10729～10736.
  • 4Strid S. FEMS Microbiology Reviews , 1999, 23: 391～406.
  • 5Liu F , Sidney A. Nucleic Acids Research, 1996, 24 (14): 2690～2696.
  • 6Diane K, Wang J , Yan Y , et al. RNA , 1998, 4: 1397～1406.
  • 7何华坤,李月琴,王波,张文军,苏运贞,周天鸿.RNase P对人巨细胞病毒mRNA的体外靶定切割研究[J].中国优生与遗传杂志,2004,12(2):8-10. 被引量：2
  • 8韩俊,李艳秋,姜永中,余莉,王明丽.人巨细胞病毒cDNA文库的构建、鉴定及pp65阳性克隆筛选[J].微生物学通报,2004,31(2):87-90. 被引量：3
  • 9Zhou T, Kim J, Ahmed F, et al. J of Biol Chem, 2002, 277 (33): 30112～30120.
  • 10Phong T, Amy W, Liu F. Nucleic Acids Research, 1999 , 27 (23) : 4590～4597.

二级参考文献18

  • 1Trang P, Lee M, Nepomuceno E, Kim J, Zhu H, Liu F. Effective inhibition of human cytomegalovirus gene expression and replication by a ribozyme derived from the catalytic RNA subunit of RNase P from Escherichia coli[J]. Proc Natl Acad Sci U S A, 2000 May,
  • 2Markus Wagner, Detlef Michel, Peter Schaarschmidt, et al. Comperison between Human Cytomegalovirus pUL97 and Murine Cytomegalovirus (MCMV) pM97 Expressed by MCMV and Vaccinia Virus: pM97 Does Not Confer Ganciclovir Sensitivity[J]. Jof Virology, November 2
  • 3Yow MD, White NH, Taber LH, et al. Acquisition of cytomegalovirus infection from birth to 10 years: a longitudinal serologic study[J]. J Pediatr, 1987, 110:37 - 42.
  • 4Joanna Kufel. Different cleavage sites are aligned differently in the active site of M1 RNA, the catalytic subunit of Escherichia coli RNase P[J]. Proc. Natl. Acad. Sci. USA, June 1996, (93) :6085- 6090.
  • 5Stinki MF, Malone CL, Hermiston TW, Liu B. Regulation of human Cytomegalovirus transplation[ M]. Boca Raton: CRC Press, 1991,245 - 260.
  • 6Fenyong Liu, RNase P- mediated inhibition of cytomegalovirus protease expression and viral DNA encapsidation by oligonucleotide external guide sequences [ J ]. PNAS, December 18, 2001, 98 (26):14831 - 14836.
  • 7Yan Yuan. Targeted cleavage of mRNA by human RNase P[J],Proc. Natl. Acad. Sci. USA, September 1992, (89) :8006 - 8010.
  • 8N. Kyle Tanner, Ribozymes: the characteristics and properties of catalytic RNAs[J]. FEMS Microbiology Reviews, 1999, (23) :257- 275.
  • 9Diane Kawa and Fenyong LIU, Inhibition of viral gene expression by human ribonuclease P[J]. RNA, 1998, (4): 1397- 1406.
  • 10Debora. Effective inhibition of influenza virus production in cultured cells by external guide sequences and ribonuclease P[J]. Proc. Natl.Acad. Sci. USA, June 1998, (95) :7327 - 7332.

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微生物学通报
2005年 第2期

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