骨形成蛋白-2表达载体的构建及其转录活性测定

Construction and Transcriptional Activity of BMP-2 Expression Vector

将骨形成蛋白-2(BMP-2)基因克隆入带HA标签的BMP-2表达载体中,得到带HA标签的重组质粒pGBKT7/HA-BMP-2,将该重组质粒转染酵母AH109、Y187细胞,Western印迹检测表明表达了与预期相对分子质量大小一致的BMP-2。BMP-2表达载体的成功构建为深入研究骨形成蛋白-2在促进骨痂生成过程中的分子机制及其生物学功能打下了基础。

To explore the molecular mechanisms of bone morphology protein-2(BMP-2), the pGBKT7/HA-BMP-2 recombinant plasmid, an BMP-2 expression vector with HA epitope, was constructed. The recombinant plasmids were transformed into yeast cell AH109 and Y187. The expression was identified by Western blot. The successful construction of the BMP-2 expression vector lays solid foundations for further study on its biological role.