霍乱毒素及视神经植块对视网膜节细胞存活的影响

The Effects of Cholera Toxin and Optic Nerve Graft on Survival of Retinal Ganglion Cells After Axotomy in Adult Hamsters

目的 探讨霍乱毒素及视神经植块对视神经切断后视网膜节细胞存活的影响。方法 研究采用荧光逆行示踪标记法和定量解剖学技术。结果 ( 1 )切断视神经 5、7及 1 4天后 ,视网膜节细胞平均密度分别为 1 1 93.8± 94.2 mm2 、846.9± 5 5 .6 mm2 及 2 1 7.5± 40 .3 mm2 。 ( 2 )给予霍乱毒素组在 5d、7d及 1 4d ,视网膜节细胞的密度分别为 1 386.3± 88.0 mm2 ,1 1 96.9± 75 .2 mm2 及 396.3± 72 .4 mm2 ,与切断视经组相比在各时间段差异均有显著性 (P <0 .0 5 )。 ( 3)玻璃体植入视神经组在 5天、7天及 1 4天视网膜节细胞的密度分别为 1 367.4± 90 .8 mm2 ,1 1 40 .6± 83.5 mm2 及 2 38.8± 37.3 mm2 ,与对照组相比 ,在 5天及 7天组差异有显著性 (P <0 .0 5 ) ,而 1 4天组差异无显著性 (P >0 .0 5 )。 ( 4 )联合给予霍乱毒素及视神经植块 ,在 5、7、1 4天视网膜节细胞的平均密度分别为 1 660 .6± 93.1 mm2 ,1 385 .6± 94.1 mm2及 5 5 6.4± 93.1 mm2 ,与神经切断组及单纯给予霍乱毒素或视神经组相比 ,差异均有显著性 (P <0 .0 5 )。结论 ( 1 )CTx和视神经植块都分别能促进受损视网膜细胞存活。 ( 2 )联合应用CTx和视神经植块 。

Objective To investigate the effects of cholera toxin (CTx) and optic nerve (ON) graft on survival of axotomied retinal ganglion cells (RGCs). Method The flourescent retrograde tracing method and quantitative anatomical techniques were used. Results (1) 5,7 and 14 days after axotomy, the mean densities of surviving RGCs were 1193.8±94.2/mm 2,846.9±55.6/mm 2 and 217.5±40.3/mm 2 respectively.(2)In the group treated with CTx ,5,7 and 14 days after axotomy,the mean densities of surviving RGCs were 1386.3±88.0/mm 2,1196.9±75.2/mm 2 and 396.3±72.4/mm 2 respectively.They were significantly different from the axotomized group(P<0.05). (3) In the group treated with an intravitreat ON graft,in 5,7 and 14 days after axotomy,the mean densities of surviving RGCs were 1367.4±90.8/mm 2,1140.6±83.5/mm 2 and 238.8±37.3mm 2 respectively,which were significantly different from the axotomized group in 5 and 7 days(P<0.05)while they weren't significantly different in 14 days(P>0.05).(4)In the groups treated with CTx plus ON graft, the mean densities of surviving RGCs were 1660.6±93.1/mm 2,1385.6±94.1/mm 2 and 556.4±93.1/mm 2 respectively,which were significantly higher than that in the axotomized group,ON graft group and CTx group. Conclusion (1) Both CTx and ON graft can promote the survival of axotomized RGCs. (2) Combination of CTx and ON graft applied into vitreous can significantly enhance the survival of axotomized RGCs.