CH50多肽在膀胱癌细胞系BIU-87中的表达和鉴定

Expression and identify of CH50 polypeptide in bladder cancer cell line BIU-87

目的:研究CH50多肽真核表达载体pCH510体外转染膀胱癌细胞系BIU87以及CH50多肽的表达和鉴定。方法:以脂质体LipofectimineTM2000为介导,将pCH510质粒体外转染给BIU87细胞,用免疫组化S P法、WesternBlot法鉴定CH50多肽的表达,RT PCR法鉴定导入基因的表达。结果:转染pCH510质粒的BIU87细胞明显阳性表达CH50多肽,对照组则不表达。结论:BIU87细胞体外转染pCH510质粒后能表达CH50多肽,改变了癌细胞的黏附属性,为临床治疗膀胱癌提供了新的途径。

Objective: To investigate the expression and identification of CH50 polypeptide in bladder cancer cell line BIU-87 by gene transfection. Methods:The eukaryotic expressing vector pCH510 of polypeptide CH50 was introduced into BIU-87 cells by gene transfection Lipofectimine TM2000.The expressed product was identified by immunohistochemistry and Western blot method. The expression of the transfected gene was identified by RT-PCR. Results:The BIU-87 cells could produce CH50 polypeptide by transfection. Conclusion:When the vector of pCH510 was transfected into BIU-87 cells in vitro, the cell adhension characteristic would be changed.