模拟潜水过程中兔血管内皮细胞的变化

Change of marker and morphology of vascular endothelium induced by simulating diving condition

目的:观察模拟潜水前、后血管内皮标记物及血管内皮细胞形态的变化。方法:14只新西兰白兔,在0.55MPa下停留35min,再升至0.7MPa停留35min,阶段减压出舱,检测加压前与减压后血浆内皮素1(ET1)和第Ⅷ因子(vWF),比较加减压前后的变化;电镜下观察阶段减压后血管内皮细胞形态变化。结果:减压后较加压前血浆ET1水平由(1.33±0.23)ng/L增至(2.99±0.35)ng/L,vWF活性由(2.35±0.47)%降至(1.89±0.34)%;减压后部分血管内皮细胞肿胀、脱落。结论:加减压过程能造成血管内皮的损伤。

Objective: To investigate the change of functional marker and morphology of vascular endothelium induced by simulating diving condition. Methods:14 rabbit were put in the chamber. They stayed for 35 min under 0.55 MPa, and another 35 min under 0.7 MPa, then decompressed to 0.1 Mpa following a stage decompression schedule. Blood sample were draw at 30 min before compression and after decompression for testing endothelin-1(ET-1) and von Willebrand factor (vWF). These parameters were compared to see the change from pre-compression to post-decompression. The morphological change of vascular endothelium was observed under electrical microscope after decompression. Results: After decompression, plasma levels of ET-1 was increased from (1.33±0.23)ng/L to (2.99± 0.35)ng/L and activity of vWF was reduced from (2.35±0.47)% to (1.89±0.34)%. Swelling and defluvium of vascular endothelium was found under electrical microscope. Conclusion: Compression-decompression can cause the damage of vascular endothelium.