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促红细胞生成素对大鼠心肌缺血-再灌注损伤的保护作用 被引量：18

Effects of erythropoietin on myocardial ischemia-reperfusion injury in rats
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摘要 目的:探讨促红细胞生成素(EPO)对大鼠心肌缺血再灌注损伤(MIRI)的保护作用及其机制。方法:采用结扎左冠状动脉前降支30min、再灌注120min的方法建立大鼠MIRI模型。将50只SD大鼠随机分为假手术组、缺血再灌注组、EPO100U/kg治疗组、EPO1000U/kg治疗组、EPO5000U/kg治疗组5组,每组10只。连续监测肢体Ⅱ导联心电图记录再灌注时心律失常情况;测定再灌注末血清磷酸肌酸激酶同工酶(CK MB),心肌丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH PX)、过氧化氢酶(CAT)、Na+K+ATP酶和Ca2+ATP酶的变化;观察心肌超微结构改变。结果:EPO显著降低再灌注室性心律失常的发生;减少再灌注末血清CK MB和心肌MDA含量,提高心肌GSH PX、CAT、Na+K+ATP酶和Ca2+ATP酶的活性;明显减轻缺血再灌注对心肌超微结构的损伤。对心肌SOD活性无影响。结论:EPO对大鼠MIRI有明显的保护作用,其机制可能与减轻氧自由基及钙超载损害有关。 Objective: To explore the effects of erythropoietin on myocardial ischemia- reperfusion injury in rats. Methods: The left anterior descending coronary artery was ligated for 30 minutes and then loosed for 120 minutes to establish the rat model of myocardial ischemia-reperfusion injury. 50 SD rats were randomly divided into five groups: sham-operation,ischemia-reperfusion,EPO 100 U/kg,EPO 1000 U /kg and EPO 5000 U/kg. During the processes, lead Ⅱ ECG was traced continuously to note the arrhythmia caused by reperfusion;the levels of serum creatine phosphokinase isoenzyme (CK-MB),myocardial malonaldehyole (MDA) 、superoxide dismutase (SOD)、glutathione peroxidase ( GSH-PX)、catalase (CAT)、Na + -K +-ATPase and Ca 2+-ATPase were detected at reperfusion 120 min. And the changes of myocardial ultrastructures were observed. Results: Erythropoietin significantly reduced the incident of ventricle arrhythmia caused by reperfusion;reduced serum CK-MB and myocardial MDA content ,enhanced the activity of myocardial GSH-PX,CAT Na +-K +-ATPase,Ca 2+-ATPase at reperfusion 120 min; markedly attenuated injury of the myocardial ultrastructures caused by the ischemia- reperfusion. Erythropoietin did not change the activity of myocardial SOD. Conclusion: Erythropoietin has protective effects on myocardial ischemia- reperfusion injury in rats,and the mechanism may be related to relieving the injury caused by oxygen free radical and calcium overload.
作者 王砚青 江时森 乐美兆 邱红
机构地区 南京大学医学院临床学院(南京军区南京总医院)心血管内科 解放军第八一医院病理科
出处 《医学研究生学报》 CAS 2005年第4期338-342,共5页 Journal of Medical Postgraduates
关键词 促红细胞生成素 再灌注损伤 心肌超微结构 大鼠 Erythropoietin Reperfusion injury Myocardial ultrastructure Rats
分类号 R541 [医药卫生—心血管疾病]
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参考文献11

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二级参考文献12

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共引文献15

同被引文献206

引证文献18

二级引证文献86

医学研究生学报
2005年 第4期

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