孕鼠暴露于可卡因对子代学习与记忆的影响

Effects of prenatal cocaine exposure on offspring' learning and memory

目的:建立妊娠中晚期暴露于可卡因的小鼠动物模型,研究可卡因对子代学习和记忆功能的影响。方法:运用水迷宫方法观察可卡因对子鼠空间辨别能力的影响;采用甲苯胺蓝染色技术观察额叶皮质及海马的发育情况。结果:可卡因可引起子代寻找平台的潜伏期延长、空间辨别能力下降;可卡因组子鼠额叶皮质神经元发育不良,层次结构不清;其海马锥体细胞极性紊乱,发育不良。结论:妊娠中晚期暴露于可卡因可引起子代额叶皮质及海马发育异常,这在可卡因引起子代空间辨别能力下降的发病机制中起着重要作用。

Objective:To study the effects of prenatal cocaine exposure on offspring' learning and memory by building a murine model. Methods:To research the effects of prenatal cocaine exposure on spatial resolution in the water maze test and observe the development of frontal cortex and hippocampus by toluidine blue staining. Results:It was found that prenatal cocaine exposure induced polarity disorder and poor development of frontal cortex and hippocampus of offspring. Water maze test showed that prenatal cocaine exposure led to extend the latency of finding platform. Conclusion:Prenatal cocaine exposure can result in abnormal development of hippocampus and frontal cortex of offspring, which may play an important role on cocaine-induced impairment of spatial resolution capacity.