脱钙骨基质复合牛骨形态发生蛋白修复兔桡骨缺损实验的放射学评价

Roentgenographic evaluation of repairing rabbit's radial segmental defect with transplantation of a demineralized bone matrix and bBMP composite

目的:对异体脱钙骨基质复合BMP修复节段性骨缺损能力进行放射学评价。方法:64只新西兰大白兔采用桡骨15mm节段性骨缺损模型,随机分为4组,A组植入异体脱钙骨基质(Demineralized Bone Matrix,DBM)与牛骨形态发生蛋白(Bovine Bone Morphogentic Protein,bBMP)复合材料,B组植入异体DBM,C组植入异体骨粒,D组为空白对照组。术后4w、8w、12w、16w,进行放射学检查和相应的组织学检查。结果:异体DBM与bBMP复合材料组X线和组织学检查显示骨愈合修复情况要明显优于异体DBM组、异体骨粒组和空白对照组。结论:DBM经bBMP复合后,可提高修复骨缺损的能力,是一种较为理想、具有高效成骨活性的植骨材料。

Objective: To evaluate roentgenographically the effect of a demineralized bone matrix and bBMP composite for repairing segmental bone defect. Methods:Sixty-four rabbits, of which four groups were divided with a blinded and randomized method, were underwent unilateral radial osteotomies to create a 1.5 cm bone defect. group A received transplantation of a demineralized bone matrix and bBMP(10mg) composite, group B demineralized bone matrix one, group C bone allograft and group D was a control group. The bone healing of every group was examined through radiographic analysis at four, eight, twelve and sixteen weeks. Results: X- ray and histological examinations demonstrated that the bone healing of DBM+ bBMP group was better than that of other groups. Conclusions: DBM composed with bBMP can enhance ability of repairing bone defect and is an ideal and effective implant substitute.