5-氟尿嘧啶和柳氮磺胺吡啶对胰腺癌细胞株BxPC-3的抗增殖作用

The anti-proliferative influence produced by 5-Fluouracil in combination with sulfasalazine on human pancreatic carcinoma cell line BxPC-3

目的探讨5-氟尿嘧啶(5-Fu)联合柳氮磺胺吡啶(sulfasalazine)对细胞株BxPC-3抗增殖作用。方法噻唑蓝(MTT)比色法、流式细胞仪、相差显微镜分别检测不同浓度(12.5、25.0、50.0、100.0)mg/L5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3生长抑制率、细胞周期、凋亡率及细胞形态变化。结果不同浓度5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3生长抑制率呈时间、剂量依赖性;100mg/L5-Fu联合0.5mmol/Lsulfasalazine作用细胞株BxPC-3凋亡率从63%(12h)增加到93%(48h),与对照组比较[t=48.87(12h),263.15(48h),P<0.01];G0/G1期细胞从80%(12h)到97%(48h),与对照组比较[t=5.97(12h),10.39(48h),P<0.01];相差显微镜观察两者联合作用12h大量细胞固缩变圆,随时间延长胞体萎缩裂解。结论两者联合作用细胞株BxPC-3有协同抑制作用,与细胞周期、凋亡率及形态变化相关。

Objective To explore the anti-proliferative effect induced by 5-Fu in combination with sulfasalazine on human pancreatic carcinoma cell line BxPC-3.Methods The growth inhibition effect,cell cycle and cell morphological changes were evaluated by MTT method,flow cytometry and phase-contrast microscopy in the presence of different concentrations of 5-Fu combined with 0.5 mmol/L sulfasalazine,respectively.Results Different concentrations of 5-Fu in combination with 0.5 mmol/L sulfasalazine inhibited the growth of cell BxPC-3 in a dose-,time-dependent manner.After treatment with 100 mg/L 5-Fu in combination with 0.5 mmol/L sulfasalazine,apoptotic rate was increased from 63% (12 h) to 93% (48 h) [t= 48.87 (12 h), 263.15 (48 h) as compared with control group (P< 0.01)]. Cells in G_0/G_1 phase were increased from 80% (12 h) to 97% (48 h) [t= 5.97 (12 h), 10.39 (48 h) as compared with control group (P< 0.01)]. Major cells shrunk in 12 h under phase-contrast microscopy,and cell body became atrophic and split with prolonged time.Conclusion Synergic repression produced by 5-Fluouracil in combination with sulfasalazine on cell BxPC-3 was correlated with cell cycle,apoptotic rate,and cell morphological changes.