白细胞介素-18与一氧化氮在非小细胞肺癌的生长与转移中的意义

Implication of serum levels of interleukin-18 and nitric oxide in tumor growth and metastasis of non-small cell lung cancer

目的探讨白细胞介素(IL)-18和一氧化氮(NO)在非小细胞肺癌(NSCLC)的生长与转移方面的影响作用。方法采用酶联免疫吸附法(ELISA)及Griess反应分别检测82例NSCLC肿瘤组与20例正常对照组血清IL-18和亚硝酸盐+硝酸盐浓度。结果肿瘤组血清IL-18浓度为(334.2±31.0)ng/L,对照组为(151.3±22.0)ng/L,肿瘤组亚硝酸盐+硝酸盐浓度为(237.1±21.0)μmol/L,对照组为(44.2±15.0)μmol/L,肿瘤组明显高于对照组。肿瘤组外周血血清IL-18和亚硝酸盐+硝酸盐浓度与患者的性别、年龄、病理类型无关。IL-18血清浓度与肿瘤的TNM分期、淋巴结转移及远处转移有关但与淋巴结转移的数量和部位及远处转移的脏器类别差异无统计学意义。而亚硝酸盐+硝酸盐浓度则与之无关。IL-18与亚硝酸盐+硝酸盐浓度之间无关联。结论研究提示血清中IL-18和亚硝酸盐+硝酸盐浓度在NSCLC患者对疾病的评估具有临床检测意义。

Objective To study the effect of IL-18 and nitric oxide (NO) on the growth and metastasis of non-small cell lung cancer (NSCLC).Methods Serum IL-18 and nitrate and nitrite levels in 82 patients with NSCLC and 20 healthy control subjects were measured by using ELISA and Griess.Results The levels of serum IL-18 were (334.2± 31.0) ng/L in NSCLC patients and (151.3± 22.0) ng/L in control subjects,respectively.The levels of nitrate and nitrite were (237.1± 21.0) μmol/L in NSCLC patients and (44.2± 15.0) μmol/L in control subjects,respectively.They were increased significantly in NSCLC patients as compared with control subjects.The levels of serum IL-18 and nitrate and nitrite were not related with age,gender,histological types in patients with NSCLC.The levels of serum IL-18 was closely associated with TNM stage,lymph node metastasis and distal metastasis,but not with its degree and organ types of metastasis.There was a negative correlation between the levels of serum IL-18 and nitrate and nitrite.Conclusion Serum IL-18 and nitrate and nitrite levels may be useful to evaluate the prognosis of the patients with NSCLC.