大鼠认知电位类P3变化与损伤中枢去甲肾上腺素能系统的关系(英文)

Damage to the central noradrenergic system and changes of P3-like cognitive potential in rats

背景:中枢去甲肾上腺素参与了学习记忆过程,但关于损毁中枢去甲肾上腺素能系统对大鼠认知电位类P3的影响报道较少。目的:研究中枢去甲肾上腺素能系统损伤对大鼠行为及认知电位类P3的影响。设计:以实验动物为研究对象的随机对照实验。单位:一所大学医学院的生理教研室。材料:选取SD雄性大鼠30只,清洁级,由中山医科大学实验动物中心提供(合格证号:26-99A018),正常条件饲养,自由饮水,定时喂食,体质量500～600g。对所有大鼠进行迷宫训练,筛选出达到学会标准的大鼠(学习记忆正常鼠)24只,随机分成空白对照组、6羟多巴胺注射组、生理盐水对照组,8只/组。干预:通过对双侧齿状回背去甲肾上腺素束注射6羟多巴胺,建立中枢去甲肾上腺素能系统损伤大鼠模型,在建模前后分别进行迷宫测试和类P3检测。主要观察指标:①主要结局:中枢去甲肾上腺素能系统损伤大鼠建模前后的类P3潜伏期,错误反应次数,全天总反应时间。②次要结局:建模后第12天类P3的测定结果。结果:和空白对照组相比,中枢去甲肾上腺素能系统的损伤大鼠类P3潜伏期和全天总反应时间显著延长,而错误反应次数则明显增多,差异均有显著性意义(P<0.05)。结论:中枢去甲肾上腺素参与动物的学习、记忆活动,并且在类P3的产生与整合中起一定作用。

BACKGROUND:It is well known that central noradrenergic system takes part in learning and memory,but there are a few researches focused on the effects of impairing the central noradrenergic system on rat's P3 like cognitive potential. OBJECTIVE:To study the effects of impairing the central noradrenergic system on rat's P3 like cognitive potential. DESIGN:Randomized controlled study based on the experimental animals. SETTING:Department of physiology in a medical college of a university. MATERIALS:Totally 30 male SD rats,clean degree,weighing from 500 to 600 g,were selected from the Experimental Animal Center of Sun Yat sen Medical University(certificate:26 99A018),drank freely and fed timely under the normal condition.After Y maze test of all the rats,24 rats with normal learning and memory function,which met the criteria specified in the description of Y maze test,were randomly divided into three groups(8 rats in each group):control group,6 oxidopamine(6 OHDA) injection group and saline control group. INTERVENTIONS:The models of central noradrenergic system impairment were made by injecting 6 hydroxysopamine(6 OHDA) into the bilateral dorsal noradrenergic bundle in dentate granule(DG).Then Y type maze test and elicitation of P3 like were carried out respectively before and after the models were built. MAIN OUTCOME MEASURES:Main outcome:P3 like latency,total reaction time(TRT) and error number(EN).Secondary outcome:Results of P3 like test recorded in the 12 days after the models were built. RESULTS:As compared with the controls,the model rats' P3 like latency and total reaction time(TRT) prolonged and error number(EN) increased statistically(P< 0.05). CONCLUSION:Central NE takes part in the activities of learning and memory and plays a certain role in the production and integration of P3 like.